To explore the structural basis for AIDS virus recognition by CD8+ lymphocytes, we sought to determine whether there is a diverse or restricted usage of T-cell receptors (TCR) by simian immunodeficiency virus of macaques (SIVmac) Gag-specific cytotoxic T lymphocytes (CTL) in the rhesus monkey. Six Gag-specific CTL clones were independently generated from an SIVmac-infected rhesus monkey. All six CTL clones recognized a single SIVmac Gag peptide in association with a single major histocompatibility complex class I gene product, Mamu-A*01. TCR α-chain sequences from these six CTL clones employed four different Vα families and five different Ja gene segments. In contrast, five of the six CTL clones expressed Vβ genes that were members of the same family, a human Vβ23 homolog. Furthermore, only one Jβ gene was expressed by four of the six CTL clones. These results indicate that TCR of SIVmac Gag-specific CTL from a rhesus monkey can exhibit a restricted usage of Vβ gene families and Jβ genes.
ASJC Scopus subject areas
- Insect Science