Pre-fusion events of sperm-oocyte interaction in the marine shrimp, Sicyonia ingentis

Wallis H. Clark, Fred J. Griffin, Athula H. Wikramanayake

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.

Original languageEnglish (US)
Pages (from-to)225-231
Number of pages7
JournalSeminars in Developmental Biology
Volume5
Issue number4
DOIs
StatePublished - Aug 1 1994
Externally publishedYes

Keywords

  • cell-cell recognition/egg/fertilization/sperm

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