Abstract
The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.
| Original language | English |
|---|---|
| Pages (from-to) | 225-231 |
| Number of pages | 7 |
| Journal | Seminars in Developmental Biology |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 1994 |
| Externally published | Yes |
Fingerprint
Keywords
- cell-cell recognition/egg/fertilization/sperm
Cite this
Pre-fusion events of sperm-oocyte interaction in the marine shrimp, Sicyonia ingentis. / Clark, Wallis H.; Griffin, Fred J.; Wikramanayake, Athula.
In: Seminars in Developmental Biology, Vol. 5, No. 4, 01.08.1994, p. 225-231.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pre-fusion events of sperm-oocyte interaction in the marine shrimp, Sicyonia ingentis
AU - Clark, Wallis H.
AU - Griffin, Fred J.
AU - Wikramanayake, Athula
PY - 1994/8/1
Y1 - 1994/8/1
N2 - The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.
AB - The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.
KW - cell-cell recognition/egg/fertilization/sperm
UR - http://www.scopus.com/inward/record.url?scp=0001164863&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001164863&partnerID=8YFLogxK
U2 - 10.1006/sedb.1994.1030
DO - 10.1006/sedb.1994.1030
M3 - Article
AN - SCOPUS:0001164863
VL - 5
SP - 225
EP - 231
JO - Seminars in Developmental Biology
JF - Seminars in Developmental Biology
SN - 1044-5781
IS - 4
ER -