TY - JOUR
T1 - Pre-fusion events of sperm-oocyte interaction in the marine shrimp, Sicyonia ingentis
AU - Clark, Wallis H.
AU - Griffin, Fred J.
AU - Wikramanayake, Athula H.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 1994/8
Y1 - 1994/8
N2 - The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.
AB - The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.
KW - cell-cell recognition/egg/fertilization/sperm
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U2 - 10.1006/sedb.1994.1030
DO - 10.1006/sedb.1994.1030
M3 - Article
AN - SCOPUS:0001164863
VL - 5
SP - 225
EP - 231
JO - Seminars in Developmental Biology
JF - Seminars in Developmental Biology
SN - 1044-5781
IS - 4
ER -