Abstract
T cell-mediated immune responses play an important role in the containment of HIV-1 replication. Therefore, an effective vaccine against HIV-1 should be able to elicit high frequencies of virus-specific CD8+ and CD4+ T cells. The highly attenuated poxvirus-based vaccine candidate, NYVAC-SIV-gag-pol-env (NYVAC-SIV-gpe), has been shown to induce and/or expand SIV-specific CD4+ and CD8+ T cell responses in both naive and infected macaques. In this study, the immunogenicity of NYVAC-SIV-gpe alone was compared with a combination regimen where priming with an optimized DNA-SIV-gag-env vaccine candidate was followed by a NYVAC-SIV-gpe boost. In macaques immunized with the prime-boost regimen, the extent and durability of CD8+ T cell response to an immunodominant SIV gag epitope was increased and these animals recognized a broader array of sub-dominant SIV epitopes in the cytolytic assay. In addition, the prime-boost regimen significantly enhanced the proliferative responses to both SIV gag and env proteins. Thus, the combination of these vaccine modalities may represent a valuable strategy in the development of a vaccine for HIV.
| Original language | English |
|---|---|
| Pages (from-to) | 7180-7191 |
| Number of pages | 12 |
| Journal | Journal of Immunology |
| Volume | 167 |
| Issue number | 12 |
| State | Published - Dec 15 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Potentiation of Simian Immunodeficiency Virus (SIV)-specific CD4+ and CD8+ T cell responses by a DNA-SIV and NYVAC-SIV prime/boost regimen. / Hel, Z.; Tsai, W. P.; Thornton, A.; Nacsa, J.; Giuliani, L.; Tryniszewska, E.; Poudyal, M.; Venzon, D.; Wang, X.; Altman, J.; Watkins, David; Lu, W.; Von Gegerfelt, A.; Felber, B. K.; Tartaglia, J.; Pavlakis, G. N.; Franchini, G.
In: Journal of Immunology, Vol. 167, No. 12, 15.12.2001, p. 7180-7191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potentiation of Simian Immunodeficiency Virus (SIV)-specific CD4+ and CD8+ T cell responses by a DNA-SIV and NYVAC-SIV prime/boost regimen
AU - Hel, Z.
AU - Tsai, W. P.
AU - Thornton, A.
AU - Nacsa, J.
AU - Giuliani, L.
AU - Tryniszewska, E.
AU - Poudyal, M.
AU - Venzon, D.
AU - Wang, X.
AU - Altman, J.
AU - Watkins, David
AU - Lu, W.
AU - Von Gegerfelt, A.
AU - Felber, B. K.
AU - Tartaglia, J.
AU - Pavlakis, G. N.
AU - Franchini, G.
PY - 2001/12/15
Y1 - 2001/12/15
N2 - T cell-mediated immune responses play an important role in the containment of HIV-1 replication. Therefore, an effective vaccine against HIV-1 should be able to elicit high frequencies of virus-specific CD8+ and CD4+ T cells. The highly attenuated poxvirus-based vaccine candidate, NYVAC-SIV-gag-pol-env (NYVAC-SIV-gpe), has been shown to induce and/or expand SIV-specific CD4+ and CD8+ T cell responses in both naive and infected macaques. In this study, the immunogenicity of NYVAC-SIV-gpe alone was compared with a combination regimen where priming with an optimized DNA-SIV-gag-env vaccine candidate was followed by a NYVAC-SIV-gpe boost. In macaques immunized with the prime-boost regimen, the extent and durability of CD8+ T cell response to an immunodominant SIV gag epitope was increased and these animals recognized a broader array of sub-dominant SIV epitopes in the cytolytic assay. In addition, the prime-boost regimen significantly enhanced the proliferative responses to both SIV gag and env proteins. Thus, the combination of these vaccine modalities may represent a valuable strategy in the development of a vaccine for HIV.
AB - T cell-mediated immune responses play an important role in the containment of HIV-1 replication. Therefore, an effective vaccine against HIV-1 should be able to elicit high frequencies of virus-specific CD8+ and CD4+ T cells. The highly attenuated poxvirus-based vaccine candidate, NYVAC-SIV-gag-pol-env (NYVAC-SIV-gpe), has been shown to induce and/or expand SIV-specific CD4+ and CD8+ T cell responses in both naive and infected macaques. In this study, the immunogenicity of NYVAC-SIV-gpe alone was compared with a combination regimen where priming with an optimized DNA-SIV-gag-env vaccine candidate was followed by a NYVAC-SIV-gpe boost. In macaques immunized with the prime-boost regimen, the extent and durability of CD8+ T cell response to an immunodominant SIV gag epitope was increased and these animals recognized a broader array of sub-dominant SIV epitopes in the cytolytic assay. In addition, the prime-boost regimen significantly enhanced the proliferative responses to both SIV gag and env proteins. Thus, the combination of these vaccine modalities may represent a valuable strategy in the development of a vaccine for HIV.
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M3 - Article
VL - 167
SP - 7180
EP - 7191
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -