Potentials of microfluorimetric techniques in basic and applied research in ophthalmology: an application to the study of photosensitizer localization in lens epithelial cells

Roberta Ramponi, Rinaldo Cubeddu, Giovanni F. Bottiroli, Jean Marie Parel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microfluorimetric techniques are nowadays widely used as a diagnostic tool in basic and applied research in biomedicine. They can be employed to study both endogenous and exogenous fluorophores. In the first case, direct indications are obtained on the biomolecules of interest, whereas in the second case indirect information can be deduced on the cellular structures interacting with the fluorophores used as the markers. Moreover, in the case of fluorescent drugs, such as most photosensitizers, microfluorimetric techniques can be exploited to determine the localization sites of the drug itself. Due to the complexity of biological systems, discrimination of the single fluorophores merely based on cw excitation and emission spectral analysis is not always possible. Thus, combination with time-resolved fluorescence analysis can give important additional indications. The potentials of these techniques in ophthalmological research are discussed. The paper reports, as an example of application of time-resolved microfluorimetric techniques, the study of the localization of Photofrin II, a widely used photosensitizing drug, in lens epithelial cells. Indeed, a photodynamic therapy of lens epithelial proliferation frequently following primary cataract surgery has recently been proposed. The potentials of this therapeutic approach strongly depend on a very efficient drug targeting. Microfluorimetric techniques proved very useful to identify the drug localization sites in a series of experiments performed using young adult rabbits as the animal model.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages193-200
Number of pages8
ISBN (Print)0819407909
StatePublished - Dec 1 1992
Externally publishedYes
EventOphthalmic Technologies II - Los Angeles, CA, USA
Duration: Jan 19 1992Jan 21 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1644
ISSN (Print)0277-786X

Other

OtherOphthalmic Technologies II
CityLos Angeles, CA, USA
Period1/19/921/21/92

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Ramponi, R., Cubeddu, R., Bottiroli, G. F., & Parel, J. M. (1992). Potentials of microfluorimetric techniques in basic and applied research in ophthalmology: an application to the study of photosensitizer localization in lens epithelial cells. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 193-200). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1644). Publ by Int Soc for Optical Engineering.