Abstract
Microfluorimetric techniques are nowadays widely used as a diagnostic tool in basic and applied research in biomedicine. They can be employed to study both endogenous and exogenous fluorophores. In the first case, direct indications are obtained on the biomolecules of interest, whereas in the second case indirect information can be deduced on the cellular structures interacting with the fluorophores used as the markers. Moreover, in the case of fluorescent drugs, such as most photosensitizers, microfluorimetric techniques can be exploited to determine the localization sites of the drug itself. Due to the complexity of biological systems, discrimination of the single fluorophores merely based on cw excitation and emission spectral analysis is not always possible. Thus, combination with time-resolved fluorescence analysis can give important additional indications. The potentials of these techniques in ophthalmological research are discussed. The paper reports, as an example of application of time-resolved microfluorimetric techniques, the study of the localization of Photofrin II, a widely used photosensitizing drug, in lens epithelial cells. Indeed, a photodynamic therapy of lens epithelial proliferation frequently following primary cataract surgery has recently been proposed. The potentials of this therapeutic approach strongly depend on a very efficient drug targeting. Microfluorimetric techniques proved very useful to identify the drug localization sites in a series of experiments performed using young adult rabbits as the animal model.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Place of Publication | Bellingham, WA, United States |
| Publisher | Publ by Int Soc for Optical Engineering |
| Pages | 193-200 |
| Number of pages | 8 |
| Volume | 1644 |
| ISBN (Print) | 0819407909 |
| State | Published - Dec 1 1992 |
| Externally published | Yes |
| Event | Ophthalmic Technologies II - Los Angeles, CA, USA Duration: Jan 19 1992 → Jan 21 1992 |
Other
| Other | Ophthalmic Technologies II |
|---|---|
| City | Los Angeles, CA, USA |
| Period | 1/19/92 → 1/21/92 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Potentials of microfluorimetric techniques in basic and applied research in ophthalmology : an application to the study of photosensitizer localization in lens epithelial cells. / Ramponi, Roberta; Cubeddu, Rinaldo; Bottiroli, Giovanni F.; Parel, Jean-Marie A.
Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1644 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1992. p. 193-200.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Potentials of microfluorimetric techniques in basic and applied research in ophthalmology
T2 - an application to the study of photosensitizer localization in lens epithelial cells
AU - Ramponi, Roberta
AU - Cubeddu, Rinaldo
AU - Bottiroli, Giovanni F.
AU - Parel, Jean-Marie A
PY - 1992/12/1
Y1 - 1992/12/1
N2 - Microfluorimetric techniques are nowadays widely used as a diagnostic tool in basic and applied research in biomedicine. They can be employed to study both endogenous and exogenous fluorophores. In the first case, direct indications are obtained on the biomolecules of interest, whereas in the second case indirect information can be deduced on the cellular structures interacting with the fluorophores used as the markers. Moreover, in the case of fluorescent drugs, such as most photosensitizers, microfluorimetric techniques can be exploited to determine the localization sites of the drug itself. Due to the complexity of biological systems, discrimination of the single fluorophores merely based on cw excitation and emission spectral analysis is not always possible. Thus, combination with time-resolved fluorescence analysis can give important additional indications. The potentials of these techniques in ophthalmological research are discussed. The paper reports, as an example of application of time-resolved microfluorimetric techniques, the study of the localization of Photofrin II, a widely used photosensitizing drug, in lens epithelial cells. Indeed, a photodynamic therapy of lens epithelial proliferation frequently following primary cataract surgery has recently been proposed. The potentials of this therapeutic approach strongly depend on a very efficient drug targeting. Microfluorimetric techniques proved very useful to identify the drug localization sites in a series of experiments performed using young adult rabbits as the animal model.
AB - Microfluorimetric techniques are nowadays widely used as a diagnostic tool in basic and applied research in biomedicine. They can be employed to study both endogenous and exogenous fluorophores. In the first case, direct indications are obtained on the biomolecules of interest, whereas in the second case indirect information can be deduced on the cellular structures interacting with the fluorophores used as the markers. Moreover, in the case of fluorescent drugs, such as most photosensitizers, microfluorimetric techniques can be exploited to determine the localization sites of the drug itself. Due to the complexity of biological systems, discrimination of the single fluorophores merely based on cw excitation and emission spectral analysis is not always possible. Thus, combination with time-resolved fluorescence analysis can give important additional indications. The potentials of these techniques in ophthalmological research are discussed. The paper reports, as an example of application of time-resolved microfluorimetric techniques, the study of the localization of Photofrin II, a widely used photosensitizing drug, in lens epithelial cells. Indeed, a photodynamic therapy of lens epithelial proliferation frequently following primary cataract surgery has recently been proposed. The potentials of this therapeutic approach strongly depend on a very efficient drug targeting. Microfluorimetric techniques proved very useful to identify the drug localization sites in a series of experiments performed using young adult rabbits as the animal model.
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M3 - Conference contribution
SN - 0819407909
VL - 1644
SP - 193
EP - 200
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Publ by Int Soc for Optical Engineering
CY - Bellingham, WA, United States
ER -