Abstract
The integral membrane glycoprotein presenilin-1 (PS1), in concert with β-secretase and beta amyloid precursor protein (βAPP), orchestrate cleavage of βAPP into amyloidogenic Aβ peptides.To gain further insight into PS1 function, we undertook a gene expression profiling study that interrogated the expression of 12 000 genes in the forebrain of PS1-hypomorphic mice that exhibit highly attenuated PS1 activity. Using stringent RNA screening, DNA array and Northern assay, we report significant down-regulation in the expression of βAPP and hypoxia inducible factor-1 alpha (HIF-1α), and a marked up-regulation in the expression of glial-specific markers that include S100β protein, glycerol phosphate dehydrogenase, and glial fibrillary acidic protein. These data suggest potential roles for PS1 in the cellular response to hypoxia and glial-specific gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 2025-2028 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 15 |
| Issue number | 13 |
| DOIs | |
| State | Published - Sep 1 2004 |
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Keywords
- Aβ peptides
- Alzheimer's disease
- Glial fibrillary acidic protein
- Gliosis
- Glycerol phosphate dehydrogenase
- Hypomorphic mice
- Hypoxia inducible factor 1 alpha
- S100β protein
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Potential roles for presenilin-1 in oxygen sensing and in glial-specific gene expression. / Cui, Jian Guo; Fraser, Paul E.; St. George-Hyslop, Peter; Westaway, David; Lukiw, Walter J.
In: NeuroReport, Vol. 15, No. 13, 01.09.2004, p. 2025-2028.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potential roles for presenilin-1 in oxygen sensing and in glial-specific gene expression
AU - Cui, Jian Guo
AU - Fraser, Paul E.
AU - St. George-Hyslop, Peter
AU - Westaway, David
AU - Lukiw, Walter J.
PY - 2004/9/1
Y1 - 2004/9/1
N2 - The integral membrane glycoprotein presenilin-1 (PS1), in concert with β-secretase and beta amyloid precursor protein (βAPP), orchestrate cleavage of βAPP into amyloidogenic Aβ peptides.To gain further insight into PS1 function, we undertook a gene expression profiling study that interrogated the expression of 12 000 genes in the forebrain of PS1-hypomorphic mice that exhibit highly attenuated PS1 activity. Using stringent RNA screening, DNA array and Northern assay, we report significant down-regulation in the expression of βAPP and hypoxia inducible factor-1 alpha (HIF-1α), and a marked up-regulation in the expression of glial-specific markers that include S100β protein, glycerol phosphate dehydrogenase, and glial fibrillary acidic protein. These data suggest potential roles for PS1 in the cellular response to hypoxia and glial-specific gene expression.
AB - The integral membrane glycoprotein presenilin-1 (PS1), in concert with β-secretase and beta amyloid precursor protein (βAPP), orchestrate cleavage of βAPP into amyloidogenic Aβ peptides.To gain further insight into PS1 function, we undertook a gene expression profiling study that interrogated the expression of 12 000 genes in the forebrain of PS1-hypomorphic mice that exhibit highly attenuated PS1 activity. Using stringent RNA screening, DNA array and Northern assay, we report significant down-regulation in the expression of βAPP and hypoxia inducible factor-1 alpha (HIF-1α), and a marked up-regulation in the expression of glial-specific markers that include S100β protein, glycerol phosphate dehydrogenase, and glial fibrillary acidic protein. These data suggest potential roles for PS1 in the cellular response to hypoxia and glial-specific gene expression.
KW - Aβ peptides
KW - Alzheimer's disease
KW - Glial fibrillary acidic protein
KW - Gliosis
KW - Glycerol phosphate dehydrogenase
KW - Hypomorphic mice
KW - Hypoxia inducible factor 1 alpha
KW - S100β protein
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UR - http://www.scopus.com/inward/citedby.url?scp=4644263024&partnerID=8YFLogxK
U2 - 10.1097/00001756-200409150-00006
DO - 10.1097/00001756-200409150-00006
M3 - Article
VL - 15
SP - 2025
EP - 2028
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 13
ER -