Potent antibody-mediated neutralization and evolution of antigenic escape variants of simian immunodeficiency virus strain SIVmac239 in vivo

Shuji Sato, Eloisa Yuste, William A. Lauer, Hyuk Chang Eun, Jennifer S. Morgan, Jacqueline G. Bixby, Jeffrey D. Lifson, Ronald Charles Desrosiers, Welkin E. Johnson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Here, we describe the evolution of antigenic escape variants in a rhesus macaque that developed unusually high neutralizing antibody titers to SIVmac239. By 42 weeks postinfection, 50% neutralization of SIVmac239 was achieved with plasma dilutions of 1:1,000. Testing of purified immunoglobulin confirmed that the neutralizing activity was antibody mediated. Despite the potency of the neutralizing antibody response, the animal displayed a typical viral load profile and progressed to terminal AIDS with a normal time course. Viral envelope sequences from week 16 and week 42 plasma contained an excess of nonsynonymous substitutions, predominantly in V1 and V4, including individual sites with ratios of nonsynonymous to synonymous substitution rates (dN/dS) highly suggestive of strong positive selection. Recombinant viruses encoding envelope sequences isolated from these time points remained resistant to neutralization by all longitudinal plasma samples, revealing the failure of the animal to mount secondary responses to the escaped variants. Substitutions at two sites with significant dN/dS values, one in V1 and one in V4, were independently sufficient to confer nearly complete resistance to neutralization. Substitutions at three additional sites, one in V4 and two in gp41, conferred moderate to high levels of resistance when tested individually. All the amino acid changes leading to escape resulted from single nucleotide substitutions. The observation that antigenic escape resulted from individual, single amino acid replacements at sites well separated in current structural models of Env indicates that the virus can utilize multiple independent pathways to rapidly achieve similar levels of resistance.

Original languageEnglish (US)
Pages (from-to)9739-9752
Number of pages14
JournalJournal of Virology
Volume82
Issue number19
DOIs
StatePublished - Oct 2008
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
neutralization
Neutralizing Antibodies
antibodies
Antibodies
neutralizing antibodies
Viruses
Amino Acids
Structural Models
Macaca mulatta
Viral Load
viruses
amino acids
Antibody Formation
Immunoglobulins
Acquired Immunodeficiency Syndrome
Nucleotides
viral load
animal behavior

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Potent antibody-mediated neutralization and evolution of antigenic escape variants of simian immunodeficiency virus strain SIVmac239 in vivo. / Sato, Shuji; Yuste, Eloisa; Lauer, William A.; Eun, Hyuk Chang; Morgan, Jennifer S.; Bixby, Jacqueline G.; Lifson, Jeffrey D.; Desrosiers, Ronald Charles; Johnson, Welkin E.

In: Journal of Virology, Vol. 82, No. 19, 10.2008, p. 9739-9752.

Research output: Contribution to journalArticle

Sato, Shuji ; Yuste, Eloisa ; Lauer, William A. ; Eun, Hyuk Chang ; Morgan, Jennifer S. ; Bixby, Jacqueline G. ; Lifson, Jeffrey D. ; Desrosiers, Ronald Charles ; Johnson, Welkin E. / Potent antibody-mediated neutralization and evolution of antigenic escape variants of simian immunodeficiency virus strain SIVmac239 in vivo. In: Journal of Virology. 2008 ; Vol. 82, No. 19. pp. 9739-9752.
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