Potassium-stimulated GABA release is a chloride-dependent but sodium- and calcium-independent process in cultured astrocytes

Jan Albrecht, Alex S. Bender, Michael D Norenberg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Depolarization of cultured astrocytes by KCl stimulated γ-aminobutyric acid (GABA) release in a dose-dependent manner. At 60 mM KCl, the stimulatory effect was calcium- and sodium- independent, and was not altered by the presence of β-alanine. The potassium-evoked GABA release was inhibited by furosemide and 4-acetamido-4'-isothiocyano-2,2'-stilbene disulfonic acid (SITS), blockers of the chloride transporter across the plasma membrane, as well by chloride ion replacement with glucuronate. Other depolarizing agents, such as veratridine and ouabain, decreased basal GABA release; ouabain also inhibited the stimulatory effect of 60 mM KCl. The high K+-induced GABA release may affect CNS excitability and may represent an important aspect of glial-neuronal interactions.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalActa Neurobiologiae Experimentalis
Volume58
Issue number3
StatePublished - Oct 1 1998

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Calcium Chloride
Sodium Chloride
Astrocytes
gamma-Aminobutyric Acid
Potassium
Ouabain
Chlorides
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Veratridine
Aminobutyrates
Stilbenes
Glucuronic Acid
Furosemide
Neuroglia
Alanine
Sodium
Cell Membrane
Ions
Calcium
Acids

Keywords

  • Calcium
  • Chloride
  • Cortical astrocytes
  • Depolarizing agents
  • GABA release
  • Sodium

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Potassium-stimulated GABA release is a chloride-dependent but sodium- and calcium-independent process in cultured astrocytes. / Albrecht, Jan; Bender, Alex S.; Norenberg, Michael D.

In: Acta Neurobiologiae Experimentalis, Vol. 58, No. 3, 01.10.1998, p. 169-175.

Research output: Contribution to journalArticle

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