Potassium rectifier currents differ in myocytes of endocardial and epicardial origin

T. Furukawa, S. Kimura, N. Furukawa, A. L. Bassett, R. J. Myerburg

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Whole-cell voltage-clamp experiments and single-channel current recordings in cell-attached patch mode were performed on enzymatically dissociated single ventricular myocytes harvested from feline endocardial and epicardial surfaces. The studies were designed to compare the characteristics of inward rectifier K+ current (IK1) and delayed rectifier K+ current (IK) between endocardial and epicardial cells and to test the hypothesis that the differential characteristics of IK1 and/or IK are responsible for the differences in action potential configuration between the two cell types. IK1 in endocardial cells displayed a distinct N-shaped current-voltage (I-V) relation, with a prominent outward current at potentials between -80 and -30 mV. In epicardial cells, an outward current region was much smaller, and the I-V relation demonstrated a blunted N-shaped I-V relation. In single-channel current recordings in cell-attached patch mode, neither unitary current amplitude of IK1 nor probability of channel opening was different between endocardial and epicardial cells, suggesting that the difference in the number of functional channels might be responsible for the differential IK1 I-V relations. The characteristics of IK also differed between endocardial and epicardial cells. The time course of growth of tail current of IK (IK,tail) (activation of IK) was significantly enhanced and that of IK,tail deactivation was delayed in epicardial cells compared with endocardial cells. The time constant of the slow component of IK activation at +20 mV was 3,950±787 msec in endocardial cells and 2,746±689 msec in epicardial cells (P<0.05); the corresponding values for IK deactivation at -50 mV were 1,041±387 msec and 1,959±551 msec, respectively (p<0.01). The voltage dependence of steady-state activation of IK,tail was similar between endocardial and epicardial cells, suggesting that the probability of channel opening at any potential was not different in the two cell types. The amplitude and density of fully activated IK (IK,full) were significantly greater in epicardial cells than in endocardial cells. At repolarization to -20 mV, IK,full amplitude was 452±113 pA in endocardial cells and 578±135 pA in epicardial cells (P<0.05), and the corresponding values for IK,full density were 2.86±0.73 and 4.21±0.83 μA/cm2, respectively (p<0.05). A nonstationary fluctuation analysis revealed that the amplitude of IK unitary current was similar between endocardial and epicardial cells (0.23±0.07 versus 0.22±0.03 pA, p=NS). Thus, the difference in whole-cell current amplitude of IK might be due to the difference in the number of functional channels per myocyte. These data lead us to suggest that the differential characteristics of IK1 and IK contribute to the difference of action potential configuration between endocardial and epicardial cells.

Original languageEnglish (US)
Pages (from-to)91-103
Number of pages13
JournalCirculation research
Volume70
Issue number1
DOIs
StatePublished - Jan 1 1992

Keywords

  • Cardiac
  • Ensemble noise analysis
  • Myocytes
  • Patch clamp
  • Potassium current

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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