Postprandial acid-base balance and ion regulation in freshwater and seawater-acclimated European flounder, Platichthys flesus

Josi R. Taylor, Jonathan M. Whittamore, Rod W. Wilson, Martin Grosell

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The effects of feeding on both acid-base and ion exchange with the environment, and internal acid-base and ion balance, in freshwater and seawater-acclimated flounder were investigated. Following voluntary feeding on a meal of 2.5-5% body mass and subsequent gastric acid secretion, no systemic alkaline tide or respiratory compensation was observed in either group. Ammonia efflux rates more than doubled from 489 ± 35 and 555 ± 64 μmol kg-1 h-1 under control conditions to 1,228 ± 127 and 1,300 ± 154 μmol kg-1 h-1 post-feeding in freshwater and seawater-acclimated fish, respectively. Based on predictions of gastric acid secreted during digestion, we calculated net postprandial internal base gains (i.e., HCO 3 - secreted from gastric parietal cells into the blood) of 3.4 mmol kg-1 in seawater and 9.1 mmol kg-1 in freshwater-acclimated flounder. However, net fluxes of ammonia, titratable alkalinity, Na+ and Cl- indicated that branchial Cl-/HCO 3 - and Na +/H+ exchange played minimal roles in counteracting these predicted base gains and cannot explain the absence of alkaline tide. Instead, intestinal Cl-/HCO 3 - exchange appears to be enhanced after feeding in both freshwater and seawater flounder. This implicates the intestine rather than the gills as a potential route of postprandial base excretion in fish, to compensate for gastric acid secretion.

Original languageEnglish
Pages (from-to)597-608
Number of pages12
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume177
Issue number6
DOIs
StatePublished - Aug 1 2007

Fingerprint

Flounder
Platichthys flesus
acid-base balance
Acid-Base Equilibrium
flounder
Seawater
Fresh Water
gastric acid
Gastric Acid
seawater
Ions
ions
Acids
ion
acid
Ammonia
tides
Tides
Fishes
ammonia

Keywords

  • Alkaline tide
  • Anion exchange
  • Bicarbonate secretion
  • Feeding
  • Osmoregulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • Physiology
  • Physiology (medical)

Cite this

Postprandial acid-base balance and ion regulation in freshwater and seawater-acclimated European flounder, Platichthys flesus. / Taylor, Josi R.; Whittamore, Jonathan M.; Wilson, Rod W.; Grosell, Martin.

In: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, Vol. 177, No. 6, 01.08.2007, p. 597-608.

Research output: Contribution to journalArticle

@article{c1c7cf867468400282fca801bd316eec,
title = "Postprandial acid-base balance and ion regulation in freshwater and seawater-acclimated European flounder, Platichthys flesus",
abstract = "The effects of feeding on both acid-base and ion exchange with the environment, and internal acid-base and ion balance, in freshwater and seawater-acclimated flounder were investigated. Following voluntary feeding on a meal of 2.5-5{\%} body mass and subsequent gastric acid secretion, no systemic alkaline tide or respiratory compensation was observed in either group. Ammonia efflux rates more than doubled from 489 ± 35 and 555 ± 64 μmol kg-1 h-1 under control conditions to 1,228 ± 127 and 1,300 ± 154 μmol kg-1 h-1 post-feeding in freshwater and seawater-acclimated fish, respectively. Based on predictions of gastric acid secreted during digestion, we calculated net postprandial internal base gains (i.e., HCO 3 - secreted from gastric parietal cells into the blood) of 3.4 mmol kg-1 in seawater and 9.1 mmol kg-1 in freshwater-acclimated flounder. However, net fluxes of ammonia, titratable alkalinity, Na+ and Cl- indicated that branchial Cl-/HCO 3 - and Na +/H+ exchange played minimal roles in counteracting these predicted base gains and cannot explain the absence of alkaline tide. Instead, intestinal Cl-/HCO 3 - exchange appears to be enhanced after feeding in both freshwater and seawater flounder. This implicates the intestine rather than the gills as a potential route of postprandial base excretion in fish, to compensate for gastric acid secretion.",
keywords = "Alkaline tide, Anion exchange, Bicarbonate secretion, Feeding, Osmoregulation",
author = "Taylor, {Josi R.} and Whittamore, {Jonathan M.} and Wilson, {Rod W.} and Martin Grosell",
year = "2007",
month = "8",
day = "1",
doi = "10.1007/s00360-007-0158-3",
language = "English",
volume = "177",
pages = "597--608",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
issn = "0174-1578",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Postprandial acid-base balance and ion regulation in freshwater and seawater-acclimated European flounder, Platichthys flesus

AU - Taylor, Josi R.

AU - Whittamore, Jonathan M.

AU - Wilson, Rod W.

AU - Grosell, Martin

PY - 2007/8/1

Y1 - 2007/8/1

N2 - The effects of feeding on both acid-base and ion exchange with the environment, and internal acid-base and ion balance, in freshwater and seawater-acclimated flounder were investigated. Following voluntary feeding on a meal of 2.5-5% body mass and subsequent gastric acid secretion, no systemic alkaline tide or respiratory compensation was observed in either group. Ammonia efflux rates more than doubled from 489 ± 35 and 555 ± 64 μmol kg-1 h-1 under control conditions to 1,228 ± 127 and 1,300 ± 154 μmol kg-1 h-1 post-feeding in freshwater and seawater-acclimated fish, respectively. Based on predictions of gastric acid secreted during digestion, we calculated net postprandial internal base gains (i.e., HCO 3 - secreted from gastric parietal cells into the blood) of 3.4 mmol kg-1 in seawater and 9.1 mmol kg-1 in freshwater-acclimated flounder. However, net fluxes of ammonia, titratable alkalinity, Na+ and Cl- indicated that branchial Cl-/HCO 3 - and Na +/H+ exchange played minimal roles in counteracting these predicted base gains and cannot explain the absence of alkaline tide. Instead, intestinal Cl-/HCO 3 - exchange appears to be enhanced after feeding in both freshwater and seawater flounder. This implicates the intestine rather than the gills as a potential route of postprandial base excretion in fish, to compensate for gastric acid secretion.

AB - The effects of feeding on both acid-base and ion exchange with the environment, and internal acid-base and ion balance, in freshwater and seawater-acclimated flounder were investigated. Following voluntary feeding on a meal of 2.5-5% body mass and subsequent gastric acid secretion, no systemic alkaline tide or respiratory compensation was observed in either group. Ammonia efflux rates more than doubled from 489 ± 35 and 555 ± 64 μmol kg-1 h-1 under control conditions to 1,228 ± 127 and 1,300 ± 154 μmol kg-1 h-1 post-feeding in freshwater and seawater-acclimated fish, respectively. Based on predictions of gastric acid secreted during digestion, we calculated net postprandial internal base gains (i.e., HCO 3 - secreted from gastric parietal cells into the blood) of 3.4 mmol kg-1 in seawater and 9.1 mmol kg-1 in freshwater-acclimated flounder. However, net fluxes of ammonia, titratable alkalinity, Na+ and Cl- indicated that branchial Cl-/HCO 3 - and Na +/H+ exchange played minimal roles in counteracting these predicted base gains and cannot explain the absence of alkaline tide. Instead, intestinal Cl-/HCO 3 - exchange appears to be enhanced after feeding in both freshwater and seawater flounder. This implicates the intestine rather than the gills as a potential route of postprandial base excretion in fish, to compensate for gastric acid secretion.

KW - Alkaline tide

KW - Anion exchange

KW - Bicarbonate secretion

KW - Feeding

KW - Osmoregulation

UR - http://www.scopus.com/inward/record.url?scp=34547125314&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547125314&partnerID=8YFLogxK

U2 - 10.1007/s00360-007-0158-3

DO - 10.1007/s00360-007-0158-3

M3 - Article

C2 - 17390137

AN - SCOPUS:34547125314

VL - 177

SP - 597

EP - 608

JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

SN - 0174-1578

IS - 6

ER -