TY - JOUR
T1 - Postnatal maturation of the kallikrein-kinin system in the rat kidney
T2 - From enzyme activity to receptor gene expression
AU - Bascands, Jean Loup
AU - Marin Castaño, Maria Encarna
AU - Bompart, Guy
AU - Pecher, Christiane
AU - Gaucher, Mireille
AU - Girolami, Jean Pierre
PY - 1996/1
Y1 - 1996/1
N2 - During the course of aging, the balance between intrarenal hormones is disturbed, These age-related changes are well documented for the vasoconstrictor renin-angiotensin system, but comparable information on the renal kallikrein-kinin system is not yet available, The status of the kallikrein-kinin system was assessed by (1) kallikrein activity, measured by RIA; (2) maximum binding site density (Bmax) and affinity (K(d)) of nonapeptide bradykinin (BK)-2 receptor, estimated by binding assays; (3) expression of BK2-receptor mRNA, detected by reverse transcription-polymerase chain reaction (RT-PCR) using specific BK2-oligonucleotide primers. These parameters were determinated on renal glomeruli of 3-, 5-, 8-, 12- and 38-wk-old normotensive rats. Kallikrein activity increased from 3.2 to 7.7 ng BK/min per mg protein. The density of BK2 binding sites also rose from 12 to 40 femtomoles/mg protein with no difference in affinity. There was no change in specificity, which remained that expected of a BK2 receptor. The increase in the density of BK2 binding sites was associated with an augmented mRNA expression, whereas p-actin mRNA used as a control remained unchanged. The ratio of BK2 mRNA to β-actin mRNA indicated maximum steady expression after 8 wk of age. The data provide evidence that the renal kallikrein-kinin system develops postnatally.
AB - During the course of aging, the balance between intrarenal hormones is disturbed, These age-related changes are well documented for the vasoconstrictor renin-angiotensin system, but comparable information on the renal kallikrein-kinin system is not yet available, The status of the kallikrein-kinin system was assessed by (1) kallikrein activity, measured by RIA; (2) maximum binding site density (Bmax) and affinity (K(d)) of nonapeptide bradykinin (BK)-2 receptor, estimated by binding assays; (3) expression of BK2-receptor mRNA, detected by reverse transcription-polymerase chain reaction (RT-PCR) using specific BK2-oligonucleotide primers. These parameters were determinated on renal glomeruli of 3-, 5-, 8-, 12- and 38-wk-old normotensive rats. Kallikrein activity increased from 3.2 to 7.7 ng BK/min per mg protein. The density of BK2 binding sites also rose from 12 to 40 femtomoles/mg protein with no difference in affinity. There was no change in specificity, which remained that expected of a BK2 receptor. The increase in the density of BK2 binding sites was associated with an augmented mRNA expression, whereas p-actin mRNA used as a control remained unchanged. The ratio of BK2 mRNA to β-actin mRNA indicated maximum steady expression after 8 wk of age. The data provide evidence that the renal kallikrein-kinin system develops postnatally.
KW - BK2 receptor
KW - Glomerulus
KW - mRNA
KW - Ontogeny
KW - PGE
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M3 - Article
C2 - 8808113
AN - SCOPUS:0030026130
VL - 7
SP - 81
EP - 89
JO - Journal of the American Society of Nephrology
JF - Journal of the American Society of Nephrology
SN - 1046-6673
IS - 1
ER -