Postnatal maturation of the kallikrein-kinin system in the rat kidney

From enzyme activity to receptor gene expression

Jean Loup Bascands, Maria Encarna Marin Castaño, Guy Bompart, Christiane Pecher, Mireille Gaucher, Jean Pierre Girolami

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

During the course of aging, the balance between intrarenal hormones is disturbed, These age-related changes are well documented for the vasoconstrictor renin-angiotensin system, but comparable information on the renal kallikrein-kinin system is not yet available, The status of the kallikrein-kinin system was assessed by (1) kallikrein activity, measured by RIA; (2) maximum binding site density (Bmax) and affinity (K(d)) of nonapeptide bradykinin (BK)-2 receptor, estimated by binding assays; (3) expression of BK2-receptor mRNA, detected by reverse transcription-polymerase chain reaction (RT-PCR) using specific BK2-oligonucleotide primers. These parameters were determinated on renal glomeruli of 3-, 5-, 8-, 12- and 38-wk-old normotensive rats. Kallikrein activity increased from 3.2 to 7.7 ng BK/min per mg protein. The density of BK2 binding sites also rose from 12 to 40 femtomoles/mg protein with no difference in affinity. There was no change in specificity, which remained that expected of a BK2 receptor. The increase in the density of BK2 binding sites was associated with an augmented mRNA expression, whereas p-actin mRNA used as a control remained unchanged. The ratio of BK2 mRNA to β-actin mRNA indicated maximum steady expression after 8 wk of age. The data provide evidence that the renal kallikrein-kinin system develops postnatally.

Original languageEnglish
Pages (from-to)81-89
Number of pages9
JournalJournal of the American Society of Nephrology
Volume7
Issue number1
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Kallikrein-Kinin System
Kidney
Gene Expression
Messenger RNA
Enzymes
Binding Sites
Actins
Bradykinin Receptors
Tissue Kallikreins
Kallikreins
DNA Primers
Bradykinin
Vasoconstrictor Agents
Renin-Angiotensin System
Reverse Transcription
Proteins
Hormones
Polymerase Chain Reaction

Keywords

  • BK2 receptor
  • Glomerulus
  • mRNA
  • Ontogeny
  • PGE

ASJC Scopus subject areas

  • Nephrology

Cite this

Bascands, J. L., Marin Castaño, M. E., Bompart, G., Pecher, C., Gaucher, M., & Girolami, J. P. (1996). Postnatal maturation of the kallikrein-kinin system in the rat kidney: From enzyme activity to receptor gene expression. Journal of the American Society of Nephrology, 7(1), 81-89.

Postnatal maturation of the kallikrein-kinin system in the rat kidney : From enzyme activity to receptor gene expression. / Bascands, Jean Loup; Marin Castaño, Maria Encarna; Bompart, Guy; Pecher, Christiane; Gaucher, Mireille; Girolami, Jean Pierre.

In: Journal of the American Society of Nephrology, Vol. 7, No. 1, 01.01.1996, p. 81-89.

Research output: Contribution to journalArticle

Bascands, JL, Marin Castaño, ME, Bompart, G, Pecher, C, Gaucher, M & Girolami, JP 1996, 'Postnatal maturation of the kallikrein-kinin system in the rat kidney: From enzyme activity to receptor gene expression', Journal of the American Society of Nephrology, vol. 7, no. 1, pp. 81-89.
Bascands, Jean Loup ; Marin Castaño, Maria Encarna ; Bompart, Guy ; Pecher, Christiane ; Gaucher, Mireille ; Girolami, Jean Pierre. / Postnatal maturation of the kallikrein-kinin system in the rat kidney : From enzyme activity to receptor gene expression. In: Journal of the American Society of Nephrology. 1996 ; Vol. 7, No. 1. pp. 81-89.
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