Postmortem changes in myofibrillar proteins of goat skeletal muscles

Nagaraj Nagathihalli, K. R. Anilakumar, K. Santhanam

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Postmortem changes at 5C in myofibrillar proteins of longissimus dorsi (LD), biceps femoris (BF), semimembranosus (SM) and semitendinosus (ST) muscles and myofibrillar structure of LD muscle of goat were investigated. Muscle samples were immediately collected after killing, and from carcasses stored at 5C for 3, 6, 9, 12 and 20 days. The sodium dodecyl sulfate-polyacry-lamide gel electrophoresis of myofibrils indicated the appearance of a 30 kDa component, depending on the type of the muscles. A new 55 kDa component appeared in BF and SM muscles during postmortem. Titin I and nebulin also disappeared during storage. The disappearance of titin 1 and nebulin and the appearance of a 30 kDa component were confirmed by Western blot analysis. The Transmission Electron Microscopy studies showed that after 3 days postmortem, Z-disks stayed unaltered. After 6 days postmortem, a little ultrastructural alteration was observed, and at 12 days postmortem a considerable degradation of Z-disk ultrastructure was shown. The Z-disk degradation, which results in the fragmentation of myofibrils and the appearance of 30 kDa components, is the major change observed in goat skeletal muscles during postmortem.

Original languageEnglish (US)
Pages (from-to)152-170
Number of pages19
JournalJournal of Food Biochemistry
Volume29
Issue number2
StatePublished - Apr 2005
Externally publishedYes

Fingerprint

Postmortem Changes
postmortem changes
myofibrillar proteins
Goats
Muscle
skeletal muscle
Skeletal Muscle
goats
Muscles
muscles
myofibrils
Connectin
Intervertebral Disc Degeneration
longissimus muscle
Myofibrils
Proteins
semitendinosus muscle
degradation
sodium dodecyl sulfate
gel electrophoresis

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Postmortem changes in myofibrillar proteins of goat skeletal muscles. / Nagathihalli, Nagaraj; Anilakumar, K. R.; Santhanam, K.

In: Journal of Food Biochemistry, Vol. 29, No. 2, 04.2005, p. 152-170.

Research output: Contribution to journalArticle

Nagathihalli, Nagaraj ; Anilakumar, K. R. ; Santhanam, K. / Postmortem changes in myofibrillar proteins of goat skeletal muscles. In: Journal of Food Biochemistry. 2005 ; Vol. 29, No. 2. pp. 152-170.
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