Postischemic Cerebral Lipid Peroxidation In Vitro: Modification by Dietary Vitamin E

Shinichi Yoshida, Raul Busto, Brant D. Watson, Mercedes Santiso, Myron D. Ginsberg

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


Using an in vitro system, we studied the effect of postischemic reoxygenation on cerebral lipid peroxidation in relation to the dietary intake of vitamin E (VE) in rats. Homogenates prepared from VE-deficient, -normal, and -supplemented brains, which were previously rendered ischemic for 30 min by decapitation, were incubated under air or nitrogen gas for 60 min. The extent of peroxidation in brain tissue was estimated by a thiobarbituric acid (TBA) test and by diene conjugation in total lipid extracts. The brain levels of α-tocopherol and of total and free fatty acids (FAs) were also determined. Aerobic incubation increased TBA reactants in all dietary groups; the effect was largest in the VE-deficient group, intermediate in the VE-normal group, and smallest in the VE-supplemented group. In contrast, nitrogen incubation did not alter the basal levels of TBA reactants except for a small rise associated with VE deficiency. Conjugated dienes changed in parallel with TBA reactants. α-Tocopherol decreased after aerobic incubation and also, to a lesser degree, after nitrogen incubation in each dietary group. Only in the reoxygenated samples of the VE-deficient group was there a significant fall in total polyunsaturated FAs. The levels of free FAs continuously increased throughout ischemia and subsequent incubation. However, the level of free polyunsaturated FAs was similar after aerobic and nitrogen incubation in each dietary group, and was not affected by VE. Thus, cerebral reoxygenation after ischemia propagates peroxidative reactions within esterified polyunsaturated FAs. The modification by VE of reoxygenation-induced lipid peroxidation suggests free radical mediation.

Original languageEnglish (US)
Pages (from-to)1593-1601
Number of pages9
JournalJournal of neurochemistry
Issue number5
StatePublished - May 1985


  • Brain ischemia
  • Fatty acids
  • Free radical peroxide
  • Rat brain
  • Vitamin E
  • α‐Tocopherol

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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