Postischemic alterations in ultrastructural cytochemistry of neuronal Golgi apparatus

Carol Petito, R. L. Lapinski

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Functional activity of the Golgi apparatus in postischemic neurons was evaluated by using thiamine pyrophosphatase (TPPase) activity as an histochemical marker for the trans cisternae. Ischemia was produced in rats by permanent occlusion of vertebral arteries and 30-minute occlusion of the carotid arteries. This insult produces irreversible ischemic injury to neurons in the striatum and CA1 zone of hippocampus but only reversible injury to neurons in the paramedian cortex and CA3 hippocampus. The number of neurons with TPPase activity in controls correlated in part with neuronal size and was found in >90% of neurons in cortex and CA3 hippocampus, 70% in CA1 hippocampus, and 40% in striatum. Ischemia plus recirculation for 30 minutes resulted in a decrease in the number of neurons with TPPase activity by 50% in CA1 hippocampus and by 80% in the three other areas. Resistant neurons in cortex and CA3 hippocampus showed partial recovery of TPPase activity by 2 hours after ischemia although the number of neurons was still less than that in controls (55% and 72%, respectively; p < 0.01). At 24 and 48 hours, TPPase activity in cortical and CA3 neurons ws similar to controls. In contrast, irreversibly injured neurons in striatum and CA1 hippocampus showed a persistent loss of TPPase activity during the entire postischemic period. Furthermore, TPPase activity remained significantly decreased in CA1 hippocampus even though previous studies in our laboratory indicated partial recovery of Golgi cisternae before subsequent cell death at 48 to 72 hours. Since TPPase activity has been correlated with functional activity within the Golgi apparatus these results suggest that glycosylation of glycoproteins and glycolipids may be markedly impaired in neurons after cerebral ischemia. The persistent abnormalities in Golgi function may contribute to the development of irreversible injury by interfering with the normal maintainence of plasma membranes and axonal transport.

Original languageEnglish
Pages (from-to)696-702
Number of pages7
JournalLaboratory Investigation
Volume55
Issue number6
StatePublished - Dec 1 1986
Externally publishedYes

Fingerprint

Histocytochemistry
Thiamine Pyrophosphatase
Golgi Apparatus
Neurons
Hippocampus
Ischemia
Wounds and Injuries
Axonal Transport
Vertebral Artery
Glycolipids
Brain Ischemia
Glycosylation
Carotid Arteries
Glycoproteins
Cell Death
Cell Membrane

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Postischemic alterations in ultrastructural cytochemistry of neuronal Golgi apparatus. / Petito, Carol; Lapinski, R. L.

In: Laboratory Investigation, Vol. 55, No. 6, 01.12.1986, p. 696-702.

Research output: Contribution to journalArticle

Petito, Carol ; Lapinski, R. L. / Postischemic alterations in ultrastructural cytochemistry of neuronal Golgi apparatus. In: Laboratory Investigation. 1986 ; Vol. 55, No. 6. pp. 696-702.
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