Post-traumatic seizures exacerbate histopathological damage after fluid-percussion brain injury

Ying Hui Bao, Helen Bramlett, Coleen M Atkins, Jessie S. Truettner, George Lotocki, Ofelia F. Alonso, W. Dalton Dietrich

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The purpose of this study was to investigate the effects of an induced period of post-traumatic epilepsy (PTE) on the histopathological damage caused by traumatic brain injury (TBI). Male Sprague Dawley rats were given a moderate parasagittal fluid-percussion brain injury (1.9-2.1 atm) or sham surgery. At 2 weeks after surgery, seizures were induced by administration of a GABA A receptor antagonist, pentylenetetrazole (PTZ, 30 mg/kg). Seizures were then assessed over a 1-h period using the Racine clinical rating scale. To evaluate whether TBI-induced pathology was exacerbated by the seizures, contusion volume and cortical and hippocampal CA3 neuronal cell loss were measured 3 days after seizures. Nearly all TBI rats showed clinical signs of PTE following the decrease in inhibitory activity. In contrast, clinically evident seizures were not observed in TBI rats given saline or sham-operated rats given PTZ. Contusions in TBI-PTZ-treated rats were significantly increased compared to the TBI-saline-treated group (p < 0.001). In addition, the TBI-PTZ rats showed less NeuN-immunoreactive cells within the ipsilateral parietal cerebral cortex (p < 0.05) and there was a trend for decreased hippocampal CA3 neurons in TBI-PTZ rats compared with TBI-saline or sham-operated rats. These results demonstrate that an induced period of post-traumatic seizures significantly exacerbates the structural damage caused by TBI. These findings emphasize the need to control seizures after TBI to limit even further damage to the injured brain.

Original languageEnglish
Pages (from-to)35-42
Number of pages8
JournalJournal of Neurotrauma
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2011

Fingerprint

Percussion
Brain Injuries
Seizures
Post-Traumatic Epilepsy
Traumatic Brain Injury
GABA-A Receptor Antagonists
Parietal Lobe
Pentylenetetrazole
Contusions
Cerebral Cortex
Sprague Dawley Rats

Keywords

  • epilepsy
  • immunohistochemistry
  • neuronal cell death
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Post-traumatic seizures exacerbate histopathological damage after fluid-percussion brain injury. / Bao, Ying Hui; Bramlett, Helen; Atkins, Coleen M; Truettner, Jessie S.; Lotocki, George; Alonso, Ofelia F.; Dalton Dietrich, W.

In: Journal of Neurotrauma, Vol. 28, No. 1, 01.01.2011, p. 35-42.

Research output: Contribution to journalArticle

Bao, Ying Hui ; Bramlett, Helen ; Atkins, Coleen M ; Truettner, Jessie S. ; Lotocki, George ; Alonso, Ofelia F. ; Dalton Dietrich, W. / Post-traumatic seizures exacerbate histopathological damage after fluid-percussion brain injury. In: Journal of Neurotrauma. 2011 ; Vol. 28, No. 1. pp. 35-42.
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