Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment

Luciana L. Farias, Débora S. Faffe, Débora G. Xisto, Maria Cristina E. Santana, Roberta Soares, Luiz Felipe M. Prota, Marcelo B. Amato, Marcelo M. Morales, Walter A. Zin, Patricia R.M. Rocco

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

This study tests the hypotheses that a recruitment maneuver per se yields and/or intensifies lung mechanical stress. Recruitment maneuver was applied to a model of paraquat-induced acute lung injury (ALI) and to healthy rats with (ATEL) or without (CTRL) previous atelectasis. Recruitment was done by using 40-CmH2O continuous positive airway pressure for 40 s. Rats were, then, ventilated for 1 h at zero end-expiratory pressure (ZEEP) or positive end-expiratory pressure (PEEP; 5 cmH2O). Atelectasis was generated by inflating a sphygmomanometer around the thorax. Additional groups did not undergo recruitment but were ventilated for 1 h under ZEEP. Lung resistive and viscoelastic pressures and static elastance were computed before and immediately after recruitment, and at the end of 1 h of ventilation. Lungs were prepared for histology. Type III procollagen (PCIII) mRNA expression in lung tissue was analyzed by RT-PCR. Lung mechanics improved after recruitment in the CTRL and ALI groups. One hour of ventilation at ZEEP increased alveolar collapse, static elastance, and lung resistive and viscoelastic pressures. Alveolar collapse was similar in ATEL and ALI, and recruitment opened the alveoli in both groups. ALI showed higher PCIII expression than ATEL or CTRL groups. One hour of ventilation at ZEEP did not increase PCIII expression but augmented it significantly in the three groups when applied after recruitment. However, PEEP ventilation after recruitment avoided any increment in PCIII expression in all groups. In conclusion, recruitment followed by ZEEP was more deleterious in ALI than in mechanical ATEL, although ZEEP alone did not elevate PCIII expression. Ventilation with 5-cmH2O PEEP prevented derecruitment and aborted the increase in PCIII expression.

Original languageEnglish (US)
Pages (from-to)53-61
Number of pages9
JournalJournal of Applied Physiology
Volume98
Issue number1
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Mechanical Stress
Positive-Pressure Respiration
Acute Lung Injury
Pressure
Ventilation
Lung
Pulmonary Atelectasis
Sphygmomanometers
Paraquat
Continuous Positive Airway Pressure
Collagen Type III
Mechanics
Histology
Thorax
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Acute lung injury
  • Procollagen expression
  • Protective ventilation strategy
  • Pulmonary mechanics

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment. / Farias, Luciana L.; Faffe, Débora S.; Xisto, Débora G.; Santana, Maria Cristina E.; Soares, Roberta; Prota, Luiz Felipe M.; Amato, Marcelo B.; Morales, Marcelo M.; Zin, Walter A.; Rocco, Patricia R.M.

In: Journal of Applied Physiology, Vol. 98, No. 1, 01.01.2005, p. 53-61.

Research output: Contribution to journalArticle

Farias, LL, Faffe, DS, Xisto, DG, Santana, MCE, Soares, R, Prota, LFM, Amato, MB, Morales, MM, Zin, WA & Rocco, PRM 2005, 'Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment', Journal of Applied Physiology, vol. 98, no. 1, pp. 53-61. https://doi.org/10.1152/japplphysiol.00118.2004
Farias, Luciana L. ; Faffe, Débora S. ; Xisto, Débora G. ; Santana, Maria Cristina E. ; Soares, Roberta ; Prota, Luiz Felipe M. ; Amato, Marcelo B. ; Morales, Marcelo M. ; Zin, Walter A. ; Rocco, Patricia R.M. / Positive end-expiratory pressure prevents lung mechanical stress caused by recruitment/derecruitment. In: Journal of Applied Physiology. 2005 ; Vol. 98, No. 1. pp. 53-61.
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AU - Santana, Maria Cristina E.

AU - Soares, Roberta

AU - Prota, Luiz Felipe M.

AU - Amato, Marcelo B.

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