Portable filter-based microdevice for detection and characterization of circulating tumor cells

Henry K. Lin, Siyang Zheng, Anthony J. Williams, Marija Balic, Susan Groshen, Howard I. Scher, Martin Fleisher, Walter Stadler, Ram Datar, Yu Chong Tai, Richard J Cote

Research output: Contribution to journalArticle

318 Citations (Scopus)

Abstract

Purpose: Sensitive detection and characterization of circulating tumor cells (CTC) could revolutionize the approach to patients with early-stage and metastatic cancer. The current methodologies have significant limitations, including limited capture efficiency and ability to characterize captured cells. Here, we report the development of a novel parylene membrane filter-based portable microdevice for size-based isolation with high recovery rate and direct on-chip characterization of captured CTC from human peripheral blood. Experimental Design: We evaluated the sensitivity and efficiency of CTC capture in a model system using blood samples from healthy donors spiked with tumor cell lines. Fifty-nine model system samples were tested to determine the recovery rate of the microdevice. Moreover, 10 model system samples and 57 blood samples from cancer patients were subjected to both membrane microfilter device and CellSearch platform enumeration for direct comparison. Results: Using the model system, the microdevice achieved >90% recovery with probability of 95% recovering at least one cell when five are seeded in 7.5 mL of blood. CTCs were identified in 51 of 57 patients using the microdevice, compared with only 26 patients with the CellSearch method. When CTCs were detected by both methods, greater numbers were recovered by the microfilter device in all but five patients. Conclusions: This filter-based microdevice is both a capture and analysis platform, capable of multiplexed imaging and genetic analysis. The microdevice presented here has the potential to enable routine CTC analysis in the clinical setting for the effective management of cancer patients.

Original languageEnglish
Pages (from-to)5011-5018
Number of pages8
JournalClinical Cancer Research
Volume16
Issue number20
DOIs
StatePublished - Oct 15 2010

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Circulating Neoplastic Cells
Equipment and Supplies
Neoplasms
Membranes
Tumor Cell Line
Research Design
Tissue Donors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Portable filter-based microdevice for detection and characterization of circulating tumor cells. / Lin, Henry K.; Zheng, Siyang; Williams, Anthony J.; Balic, Marija; Groshen, Susan; Scher, Howard I.; Fleisher, Martin; Stadler, Walter; Datar, Ram; Tai, Yu Chong; Cote, Richard J.

In: Clinical Cancer Research, Vol. 16, No. 20, 15.10.2010, p. 5011-5018.

Research output: Contribution to journalArticle

Lin, HK, Zheng, S, Williams, AJ, Balic, M, Groshen, S, Scher, HI, Fleisher, M, Stadler, W, Datar, R, Tai, YC & Cote, RJ 2010, 'Portable filter-based microdevice for detection and characterization of circulating tumor cells', Clinical Cancer Research, vol. 16, no. 20, pp. 5011-5018. https://doi.org/10.1158/1078-0432.CCR-10-1105
Lin, Henry K. ; Zheng, Siyang ; Williams, Anthony J. ; Balic, Marija ; Groshen, Susan ; Scher, Howard I. ; Fleisher, Martin ; Stadler, Walter ; Datar, Ram ; Tai, Yu Chong ; Cote, Richard J. / Portable filter-based microdevice for detection and characterization of circulating tumor cells. In: Clinical Cancer Research. 2010 ; Vol. 16, No. 20. pp. 5011-5018.
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