Porphyrin-based photocatalytic nanolithography: A new fabrication tool for protein arrays

Jane P. Bearinger, Gary Stone, Lawrence C. Dugan, Bassem El Dasher, Cheryl Stockton, James W. Conway, Tobias Kuenzler, Jeffrey A. Hubbell

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Nanoarray fabrication is a multidisciplinary endeavor encompassing materials science, chemical engineering, and biology. We formed nanoarrays via a new technique, porphyrin-based photocatalytic nanolithography. The nanoarrays, with controlled features as small as 200 nm, exhibited regularly ordered patterns and may be appropriate for (a) rapid and parallel proteomics screening of immobilized biomolecules, (b) protein-protein interactions, and/or (c) biophysical and molecular biology studies involving spatially dictated ligand placement. We demonstrated protein immobilization utilizing nanoarrays fabricated via photocatalytic nanolithography on silicon substrates where the immobilized proteins are surrounded by a non-fouling polymer background. Molecular & Cellular Proteomics 8:1823-1831, 2009.

Original languageEnglish (US)
Pages (from-to)1823-1831
Number of pages9
JournalMolecular and Cellular Proteomics
Volume8
Issue number8
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

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