Porous PTFE membranes for keratoprosthesis: Comparison of intracorneal biocompatibility

S. Gautier, B. Duchesne, J. M. Parel, E. Lacombe, J. M. Legeais, E. Alfonso

Research output: Contribution to journalReview article

3 Scopus citations

Abstract

Purpose. To investigate the biocompatibility of porous PTFE membranes used for anchoring keratoprostheses by assessing transparency, neovascularization and biocolonization in rabbits. Methods. Porous PTFE membranes: #1: 50-80μm pores, 260μm thick, 85% porosity; #2: 5-6μm pores, 115μm thick, 90% porosity; #3: 10μm pores, 130μm thick, 75% porosity; #4: 30-60μm pores, 130μm thick, 94% porosity. Ten 3 months old pigmented rabbits in 2 groups. Group I: PTFE wetted prior implantation by ethanol to BSS to autologous blood. Group II: implantation without wetting. In the right eyes, four 4mm, 80% thickness incisions were made with a diamond knife, 4 separate intrastromal pockets were delaminated and a membrane of each type inserted. No suture nor treatment were carried out. In Group I, control eyes were incised, delaminated and received autologous blood. In Group II, control eyes were incised and delaminated. Rabbits were followed by slit-lamp, photo and video. Enucleation was done at POD 7, 14 and 28 for histology and immuno-histo. Results. No corneal perforation occured. Infection occurred with 4 eyes (2 per groups), always at 6 o'clock. Membranes #1 and #4 were always the most translucent. Neovascularization took ≈ 15 days and was much higher with the blood-wetted membranes. No epithelial or AC reaction occured. Conclusion. All PTFE membranes were biocompatible and allowed neovascularization. Membrane #1 and #4 are best suited as a keratoprosthesis anchor.

Original languageEnglish (US)
Pages (from-to)S316
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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