Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering

Sayed Hadi Mirmalek-Sani, Giuseppe Orlando, John P. McQuilling, Rajesh Pareta, David L. Mack, Marcus Salvatori, Alan C. Farney, Robert J. Stratta, Anthony Atala, Emmanuel C. Opara, Shay Soker

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Emergent technologies of regenerative medicine have the potential to overcome the limitations of organ transplantation by supplying tissues and organs bioengineered in the laboratory. Pancreas bioengineering requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native extracellular matrix (ECM). We describe the generation of a whole organ, three-dimensional pancreas scaffold using acellular porcine pancreas. Imaging studies confirm that our protocol effectively removes cellular material while preserving ECM proteins and the native vascular tree. The scaffold was seeded with human stem cells and porcine pancreatic islets, demonstrating that the decellularized pancreas can support cellular adhesion and maintenance of cell functions. These findings advance the field of regenerative medicine towards the development of a fully functional, bioengineered pancreas capable of establishing and sustaining euglycemia and may be used for transplantation to cure diabetes mellitus.

Original languageEnglish
Pages (from-to)5488-5495
Number of pages8
JournalBiomaterials
Volume34
Issue number22
DOIs
StatePublished - Jul 1 2013
Externally publishedYes

Fingerprint

Bioengineering
Islets of Langerhans
Scaffolds
Extracellular Matrix
Pancreas
Swine
Transplantation (surgical)
Scaffolds (biology)
Medical problems
Stem cells
Regenerative Medicine
Adhesion
Extracellular Matrix Proteins
Blood Vessels
Tissue
Proteins
Imaging techniques
Organ Transplantation
Cell Adhesion
Diabetes Mellitus

Keywords

  • Acellular
  • Diabetes
  • Islets
  • Transplantation

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Mirmalek-Sani, S. H., Orlando, G., McQuilling, J. P., Pareta, R., Mack, D. L., Salvatori, M., ... Soker, S. (2013). Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. Biomaterials, 34(22), 5488-5495. https://doi.org/10.1016/j.biomaterials.2013.03.054

Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. / Mirmalek-Sani, Sayed Hadi; Orlando, Giuseppe; McQuilling, John P.; Pareta, Rajesh; Mack, David L.; Salvatori, Marcus; Farney, Alan C.; Stratta, Robert J.; Atala, Anthony; Opara, Emmanuel C.; Soker, Shay.

In: Biomaterials, Vol. 34, No. 22, 01.07.2013, p. 5488-5495.

Research output: Contribution to journalArticle

Mirmalek-Sani, SH, Orlando, G, McQuilling, JP, Pareta, R, Mack, DL, Salvatori, M, Farney, AC, Stratta, RJ, Atala, A, Opara, EC & Soker, S 2013, 'Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering', Biomaterials, vol. 34, no. 22, pp. 5488-5495. https://doi.org/10.1016/j.biomaterials.2013.03.054
Mirmalek-Sani SH, Orlando G, McQuilling JP, Pareta R, Mack DL, Salvatori M et al. Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. Biomaterials. 2013 Jul 1;34(22):5488-5495. https://doi.org/10.1016/j.biomaterials.2013.03.054
Mirmalek-Sani, Sayed Hadi ; Orlando, Giuseppe ; McQuilling, John P. ; Pareta, Rajesh ; Mack, David L. ; Salvatori, Marcus ; Farney, Alan C. ; Stratta, Robert J. ; Atala, Anthony ; Opara, Emmanuel C. ; Soker, Shay. / Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering. In: Biomaterials. 2013 ; Vol. 34, No. 22. pp. 5488-5495.
@article{8ac03d982ceb43258e13f2cc8f46c192,
title = "Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering",
abstract = "Emergent technologies of regenerative medicine have the potential to overcome the limitations of organ transplantation by supplying tissues and organs bioengineered in the laboratory. Pancreas bioengineering requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native extracellular matrix (ECM). We describe the generation of a whole organ, three-dimensional pancreas scaffold using acellular porcine pancreas. Imaging studies confirm that our protocol effectively removes cellular material while preserving ECM proteins and the native vascular tree. The scaffold was seeded with human stem cells and porcine pancreatic islets, demonstrating that the decellularized pancreas can support cellular adhesion and maintenance of cell functions. These findings advance the field of regenerative medicine towards the development of a fully functional, bioengineered pancreas capable of establishing and sustaining euglycemia and may be used for transplantation to cure diabetes mellitus.",
keywords = "Acellular, Diabetes, Islets, Transplantation",
author = "Mirmalek-Sani, {Sayed Hadi} and Giuseppe Orlando and McQuilling, {John P.} and Rajesh Pareta and Mack, {David L.} and Marcus Salvatori and Farney, {Alan C.} and Stratta, {Robert J.} and Anthony Atala and Opara, {Emmanuel C.} and Shay Soker",
year = "2013",
month = "7",
day = "1",
doi = "10.1016/j.biomaterials.2013.03.054",
language = "English",
volume = "34",
pages = "5488--5495",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "22",

}

TY - JOUR

T1 - Porcine pancreas extracellular matrix as a platform for endocrine pancreas bioengineering

AU - Mirmalek-Sani, Sayed Hadi

AU - Orlando, Giuseppe

AU - McQuilling, John P.

AU - Pareta, Rajesh

AU - Mack, David L.

AU - Salvatori, Marcus

AU - Farney, Alan C.

AU - Stratta, Robert J.

AU - Atala, Anthony

AU - Opara, Emmanuel C.

AU - Soker, Shay

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Emergent technologies of regenerative medicine have the potential to overcome the limitations of organ transplantation by supplying tissues and organs bioengineered in the laboratory. Pancreas bioengineering requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native extracellular matrix (ECM). We describe the generation of a whole organ, three-dimensional pancreas scaffold using acellular porcine pancreas. Imaging studies confirm that our protocol effectively removes cellular material while preserving ECM proteins and the native vascular tree. The scaffold was seeded with human stem cells and porcine pancreatic islets, demonstrating that the decellularized pancreas can support cellular adhesion and maintenance of cell functions. These findings advance the field of regenerative medicine towards the development of a fully functional, bioengineered pancreas capable of establishing and sustaining euglycemia and may be used for transplantation to cure diabetes mellitus.

AB - Emergent technologies of regenerative medicine have the potential to overcome the limitations of organ transplantation by supplying tissues and organs bioengineered in the laboratory. Pancreas bioengineering requires a scaffold that approximates the biochemical, spatial and vascular relationships of the native extracellular matrix (ECM). We describe the generation of a whole organ, three-dimensional pancreas scaffold using acellular porcine pancreas. Imaging studies confirm that our protocol effectively removes cellular material while preserving ECM proteins and the native vascular tree. The scaffold was seeded with human stem cells and porcine pancreatic islets, demonstrating that the decellularized pancreas can support cellular adhesion and maintenance of cell functions. These findings advance the field of regenerative medicine towards the development of a fully functional, bioengineered pancreas capable of establishing and sustaining euglycemia and may be used for transplantation to cure diabetes mellitus.

KW - Acellular

KW - Diabetes

KW - Islets

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=84877601258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877601258&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2013.03.054

DO - 10.1016/j.biomaterials.2013.03.054

M3 - Article

C2 - 23583038

AN - SCOPUS:84877601258

VL - 34

SP - 5488

EP - 5495

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 22

ER -