Abstract
The genetic relationships among northern South China Sea populations of the six bar wrasse (Thallasoma hardwicki) were investigated. Fish collected from the Solomon Islands were used for geographic comparison. In 1998 and 1999, a total of 100 fish were sampled from 6 localities of the northern South China Sea and 3 localities of the Solomon Islands. Genetic variations in DNA sequences were examined from the first hypervariable region (HVR-1) of the mitochondrial control region, as amplified by polymerase chain reaction. High levels of haplotypic diversity (h = 0.944 ± 0.0016, π = 0.0224 ± 0.01171) in the HVR-1 region of the mitochondrial control region of T. hardwicki were detected. This yielded 94 haplotypes that exhibited a minimum spanning tree with a starburst structure, suggestive of a very recent origin for most haplotypes. Neutrality tests indicated that the pattern of genetic variability in T. hardwicki is consistent either with genetic hitchhiking by an advantageous mutation or with population expansion. Partitioning populations into coherent geographic groups divided the northern South China Sea samples (Φ;CT = 0.0313, P < 0.001) into 3 major groups: a north-central group composed of northwestern Taiwan and northern Vietnam; a southwestern group containing southern Vietnam; and a southern group including the central Philippines. These results are in concordance with mesoscale boundaries proposed by allozyme markers, thus highlighting the importance of identifying transboundary units for the conservation and management of fisheries in the South China Sea.
| Original language | English |
|---|---|
| Pages (from-to) | 312-326 |
| Number of pages | 15 |
| Journal | Marine Biotechnology |
| Volume | 6 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2004 |
| Externally published | Yes |
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Keywords
- Demographic history
- Mitochondrial control region
- Population structure
- South China Sea
- Thallasoma hardwicki
ASJC Scopus subject areas
- Aquatic Science
- Biotechnology
- Biochemistry
Cite this
Population structure and genetic variability of six bar wrasse (Thallasoma hardwicki) in northern South China Sea revealed by mitochondrial control region sequences. / Chen, Chaolun Allen; Ablan, Maria Carmen Anonuevo; McManus, John; Bell, Johann Diepernk; Tuan, Vo Si; Cabanban, Annadel Sarmiento; Shao, Kwang Tsao.
In: Marine Biotechnology, Vol. 6, No. 4, 01.08.2004, p. 312-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Population structure and genetic variability of six bar wrasse (Thallasoma hardwicki) in northern South China Sea revealed by mitochondrial control region sequences
AU - Chen, Chaolun Allen
AU - Ablan, Maria Carmen Anonuevo
AU - McManus, John
AU - Bell, Johann Diepernk
AU - Tuan, Vo Si
AU - Cabanban, Annadel Sarmiento
AU - Shao, Kwang Tsao
PY - 2004/8/1
Y1 - 2004/8/1
N2 - The genetic relationships among northern South China Sea populations of the six bar wrasse (Thallasoma hardwicki) were investigated. Fish collected from the Solomon Islands were used for geographic comparison. In 1998 and 1999, a total of 100 fish were sampled from 6 localities of the northern South China Sea and 3 localities of the Solomon Islands. Genetic variations in DNA sequences were examined from the first hypervariable region (HVR-1) of the mitochondrial control region, as amplified by polymerase chain reaction. High levels of haplotypic diversity (h = 0.944 ± 0.0016, π = 0.0224 ± 0.01171) in the HVR-1 region of the mitochondrial control region of T. hardwicki were detected. This yielded 94 haplotypes that exhibited a minimum spanning tree with a starburst structure, suggestive of a very recent origin for most haplotypes. Neutrality tests indicated that the pattern of genetic variability in T. hardwicki is consistent either with genetic hitchhiking by an advantageous mutation or with population expansion. Partitioning populations into coherent geographic groups divided the northern South China Sea samples (Φ;CT = 0.0313, P < 0.001) into 3 major groups: a north-central group composed of northwestern Taiwan and northern Vietnam; a southwestern group containing southern Vietnam; and a southern group including the central Philippines. These results are in concordance with mesoscale boundaries proposed by allozyme markers, thus highlighting the importance of identifying transboundary units for the conservation and management of fisheries in the South China Sea.
AB - The genetic relationships among northern South China Sea populations of the six bar wrasse (Thallasoma hardwicki) were investigated. Fish collected from the Solomon Islands were used for geographic comparison. In 1998 and 1999, a total of 100 fish were sampled from 6 localities of the northern South China Sea and 3 localities of the Solomon Islands. Genetic variations in DNA sequences were examined from the first hypervariable region (HVR-1) of the mitochondrial control region, as amplified by polymerase chain reaction. High levels of haplotypic diversity (h = 0.944 ± 0.0016, π = 0.0224 ± 0.01171) in the HVR-1 region of the mitochondrial control region of T. hardwicki were detected. This yielded 94 haplotypes that exhibited a minimum spanning tree with a starburst structure, suggestive of a very recent origin for most haplotypes. Neutrality tests indicated that the pattern of genetic variability in T. hardwicki is consistent either with genetic hitchhiking by an advantageous mutation or with population expansion. Partitioning populations into coherent geographic groups divided the northern South China Sea samples (Φ;CT = 0.0313, P < 0.001) into 3 major groups: a north-central group composed of northwestern Taiwan and northern Vietnam; a southwestern group containing southern Vietnam; and a southern group including the central Philippines. These results are in concordance with mesoscale boundaries proposed by allozyme markers, thus highlighting the importance of identifying transboundary units for the conservation and management of fisheries in the South China Sea.
KW - Demographic history
KW - Mitochondrial control region
KW - Population structure
KW - South China Sea
KW - Thallasoma hardwicki
UR - http://www.scopus.com/inward/record.url?scp=11144227447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=11144227447&partnerID=8YFLogxK
U2 - 10.1007/s10126-003-0028-2
DO - 10.1007/s10126-003-0028-2
M3 - Article
C2 - 15129326
AN - SCOPUS:11144227447
VL - 6
SP - 312
EP - 326
JO - Marine Biotechnology
JF - Marine Biotechnology
SN - 1436-2228
IS - 4
ER -