Polynucleotide phosphorylase and the T3SS

Jason A. Rosenzweig, Kurt Schesser

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Low temperatures as well as encounters with host phagocytes are two stresses that have been relatively well studied in many species of bacteria. The exoribonuclease polynucleotide phosphorylase (PNPase) has previously been shown to be required by several species of bacteria, including Yersinia, for low-temperature growth. We have shown that PNPase also enhances the ability of Yersinia to withstand the killing activities of murine macrophages. We have gone on to show that PNPase is required for the optimal functioning of Yersinia's type three secretion system (T3SS), an organelle that injects effector proteins directly into host cells. Surprisingly, the PNPase-mediated effect on T3SS activity is independent of PNPase's ribonuclease activity and instead requires only its S1 RNA-binding domain. In stark contrast, the catalytic activity of PNPase is strictly required for enhanced growth at low temperature. Preliminary experiments suggest that the RNA-binding interface of the S1 domain is critical for its T3SS-enhancing activity. Our findings indicate that PNPase plays versatile roles in promoting Yersinia's survival in response to stressful conditions.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages217-224
Number of pages8
Volume603
DOIs
StatePublished - Dec 1 2007

Publication series

NameAdvances in Experimental Medicine and Biology
Volume603
ISSN (Print)00652598

Fingerprint

Polyribonucleotide Nucleotidyltransferase
Yersinia
Temperature
Bacteria
Exoribonucleases
RNA
Macrophages
Growth temperature
Phagocytes
Ribonucleases
Growth
Organelles
Catalyst activity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rosenzweig, J. A., & Schesser, K. (2007). Polynucleotide phosphorylase and the T3SS. In Advances in Experimental Medicine and Biology (Vol. 603, pp. 217-224). (Advances in Experimental Medicine and Biology; Vol. 603). https://doi.org/10.1007/978-0-387-72124-8-19

Polynucleotide phosphorylase and the T3SS. / Rosenzweig, Jason A.; Schesser, Kurt.

Advances in Experimental Medicine and Biology. Vol. 603 2007. p. 217-224 (Advances in Experimental Medicine and Biology; Vol. 603).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Rosenzweig, JA & Schesser, K 2007, Polynucleotide phosphorylase and the T3SS. in Advances in Experimental Medicine and Biology. vol. 603, Advances in Experimental Medicine and Biology, vol. 603, pp. 217-224. https://doi.org/10.1007/978-0-387-72124-8-19
Rosenzweig JA, Schesser K. Polynucleotide phosphorylase and the T3SS. In Advances in Experimental Medicine and Biology. Vol. 603. 2007. p. 217-224. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-0-387-72124-8-19
Rosenzweig, Jason A. ; Schesser, Kurt. / Polynucleotide phosphorylase and the T3SS. Advances in Experimental Medicine and Biology. Vol. 603 2007. pp. 217-224 (Advances in Experimental Medicine and Biology).
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