Polymyxin B causes coordinate inhibition of phorbol ester-induced C-kinase activity and proliferation of B lymphocytes

Andre E. Nel, Marie W. Wooten, Pascal Goldschmidt-Clermont, Paul J. Miller, Henry C. Stevenson, Robert M. Galbraith

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lymphocytes were found to be rich in phospholipid Ca2+-dependent (C-kinase) activity. Addition of polymyxin B (PMB) to in vitro assays of endogenous and exogenous phosphorylation resulted in profound inhibition of C-kinase activity. The phorbol ester 12-o-tetradecanoyl phorbol-13-acetate (TPA) directly activated C-kinase, leading to increased phosphorylation of the same substrates. TPA also stimulated proliferation of B cells as assessed by 3H-thymidine uptake, and PMB strongly inhibited this effect. This coordinate inhibition of TPA-induced phosphorylation and mitogenesis indicates that PMB is a potentially useful inhibitor of C-kinase activity, and that this enzyme may play an important role in mediating B cell responses.

Original languageEnglish
Pages (from-to)1364-1372
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume128
Issue number3
DOIs
StatePublished - May 16 1985
Externally publishedYes

Fingerprint

Polymyxin B
Lymphocytes
Phorbol Esters
Phosphorylation
B-Lymphocytes
Phosphotransferases
Acetates
Cells
Thymidine
Assays
Phospholipids
calcium ascorbate
Substrates
Enzymes
phorbol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Polymyxin B causes coordinate inhibition of phorbol ester-induced C-kinase activity and proliferation of B lymphocytes. / Nel, Andre E.; Wooten, Marie W.; Goldschmidt-Clermont, Pascal; Miller, Paul J.; Stevenson, Henry C.; Galbraith, Robert M.

In: Biochemical and Biophysical Research Communications, Vol. 128, No. 3, 16.05.1985, p. 1364-1372.

Research output: Contribution to journalArticle

Nel, Andre E. ; Wooten, Marie W. ; Goldschmidt-Clermont, Pascal ; Miller, Paul J. ; Stevenson, Henry C. ; Galbraith, Robert M. / Polymyxin B causes coordinate inhibition of phorbol ester-induced C-kinase activity and proliferation of B lymphocytes. In: Biochemical and Biophysical Research Communications. 1985 ; Vol. 128, No. 3. pp. 1364-1372.
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AU - Stevenson, Henry C.

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