Poly(ethylene oxide)-graft-poly(L-lysine) copolymers to enhance the biocompatibility of poly(L-lysine)-alginate microcapsule membranes

Amarpreet S. Sawhney, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

A graft copolymer having poly(l-lysine) (PLL) as the backbone and monomethoxy poly(ethylene glycol) (MPEG) as pendent chains was synthesized. This polycationic copolymer was used to form microcapsules with sodium alginate, a polyanion. Microcapsules and model surfaces formed with PLL-graft-MPEG demonstrated reduced protein adsorption, complement binding and cell adhesion in vitro compared to materials with unmodified PLL. Microcapsules with PLL-g-MPEG on the surface were seen to be much more biocompatible than the widely used alginate/PLL/alginate microcapsule in a mouse intraperitoneal implant model. The graft copolymers demonstrated a lower affinity for alginate and increased microcapsule permeability more than PLL. To correct this, pentalayered alginate/PLL/alginate/PLL-g-MPEG/alginate microcapsules were fabricated, and these demonstrated both appropriate permselectivity and enhanced biocompatibility.

Original languageEnglish
Pages (from-to)863-870
Number of pages8
JournalBiomaterials
Volume13
Issue number12
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Ethylene Oxide
Alginate
Phase locked loops
Polyethylene oxides
Biocompatibility
Grafts
Lysine
Capsules
Copolymers
Membranes
Ethylene Glycol
Transplants
Polyethylene glycols
Graft copolymers
Sodium alginate
alginate-polylysine-alginate
alginic acid
Cell adhesion
Cell Adhesion
Adsorption

Keywords

  • alginate
  • biocompatibility
  • graft copolymer
  • Microcapsules
  • poly(l-lysine)
  • polyethylene oxide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Poly(ethylene oxide)-graft-poly(L-lysine) copolymers to enhance the biocompatibility of poly(L-lysine)-alginate microcapsule membranes. / Sawhney, Amarpreet S.; Hubbell, Jeffrey A.

In: Biomaterials, Vol. 13, No. 12, 01.01.1992, p. 863-870.

Research output: Contribution to journalArticle

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