Poly(ethylene oxide)-graft-poly(L-lysine) copolymers to enhance the biocompatibility of poly(L-lysine)-alginate microcapsule membranes

Amarpreet S. Sawhney, Jeffrey A. Hubbell

Research output: Contribution to journalArticle

136 Scopus citations

Abstract

A graft copolymer having poly(l-lysine) (PLL) as the backbone and monomethoxy poly(ethylene glycol) (MPEG) as pendent chains was synthesized. This polycationic copolymer was used to form microcapsules with sodium alginate, a polyanion. Microcapsules and model surfaces formed with PLL-graft-MPEG demonstrated reduced protein adsorption, complement binding and cell adhesion in vitro compared to materials with unmodified PLL. Microcapsules with PLL-g-MPEG on the surface were seen to be much more biocompatible than the widely used alginate/PLL/alginate microcapsule in a mouse intraperitoneal implant model. The graft copolymers demonstrated a lower affinity for alginate and increased microcapsule permeability more than PLL. To correct this, pentalayered alginate/PLL/alginate/PLL-g-MPEG/alginate microcapsules were fabricated, and these demonstrated both appropriate permselectivity and enhanced biocompatibility.

Original languageEnglish (US)
Pages (from-to)863-870
Number of pages8
JournalBiomaterials
Volume13
Issue number12
DOIs
StatePublished - 1992

Keywords

  • alginate
  • biocompatibility
  • graft copolymer
  • Microcapsules
  • poly(l-lysine)
  • polyethylene oxide

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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