Polyclonal and antigen-specific B-Cell responses in patients with common variable immunodeficiency

Savita G. Pahwa, Michael K. Hoffman, Rajendra N. Pahwa, Robert A. Good

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Antigen-specific antibody responses were investigated in 32 hypogammaglobulinemic patients with common variable immunodeficiency following in vitro sensitization of their peripheral blood lymphocyte cultures with sheep red blood-cell determinants. Anti-sheep red blood-cell antibody-secreting cells were quantitated in a hemolytic plaque assay. Amplification of T-cell help was achieved with the use of the T-cell mitogen concanavalin A or allogeneic irradiated T cells. Four patients groups, A through D, were identified. Group A was comprised of 10 patients whose cultured lymphocyte readily developed into antibody secreting cells. Cultures of 9 patients (Group B) responded suboptimally, but were enhanced following mitogen activation of autologous or exogenous T cells, and those of 7 patients (Group C) responded only when help was aplified. In 7 patients (Group D), no responses were elicited. On the simultaneous assessment of pokeweed mitogen-driven polyclonal generation of immunoglobulin-secreting cells, only 10 responders, all from groups A and B, were identified. Our observations indicate that the majority of patients with common variable immunodeficiency possesses B cells capable of producing antibody in vitro. The ability of some patients' B cells to respond only in the antigen-specific assay while failing to do so in pokeweed mitogen-stimulated cultures suggests that these two reactions are not identical in their activation pathways.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalJournal of Clinical Immunology
Volume2
Issue number3
DOIs
StatePublished - Jul 1 1982

Keywords

  • antigen specific
  • Immunodeficiency
  • plaque forming cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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