Polychlorinated biphenyls disrupt blood-brain barrier integrity and promote brain metastasis formation

Melissa Seelbach, Lei Chen, Anita Powell, Yean Jung Choi, Bei Zhang, Bernhard Hennig, Michal J Toborek

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Background: Polychlorinated biphenyls (PCBs) comprise a ubiquitous class of toxic substances associated with carcinogenic and tumor-promoting effects as well as neurotoxic properties in the brain. However, the effects of PCBs on the development of tumor metastases are not fully understood. Objective: We evaluated the hypothesis that exposure to individual PCB congeners can facilitate the development of brain metastases in immunocompetent mice via the disruption of the integrity of the blood-brain barrier (BBB). Methods: C57/Bl6 mice were exposed to individual PCBs by oral gavage, and 48 hr later they were injected with luciferase-labeled K1735 M2 melanoma cells into the internal carotid artery. The development of metastatic nodules was monitored by bioluminescent imaging. In addition, we evaluated the functional permeability of the BBB by measuring permeability of sodium fluorescein across the brain microvessels. Expression and colocalization of tight junction (TJ) proteins were studied by Western blotting and immunofluorescence microscopy. Results: Oral administration of coplanar PCB126, mono-ortho-substituted PCB118, and non-coplanar PCB153 (each at 150 μmol/kg body weight) differentially altered expression of the TJ proteins claudin-5, occludin, and zonula occludens-1 in brain capillaries. These alterations were associated with increased permeability of the BBB. Most importantly, exposure to individual PCB congeners enhanced the rate of formation and progression of brain metastases of luciferase-tagged melanoma cells. Conclusions: Our results show for the first time that exposure to individual PCBs can facilitate the formation of bloodborne metastases via alterations of the integrity of the brain capillary endothelium.

Original languageEnglish
Pages (from-to)479-484
Number of pages6
JournalEnvironmental Health Perspectives
Volume118
Issue number4
DOIs
StatePublished - Apr 1 2010
Externally publishedYes

Fingerprint

Polychlorinated Biphenyls
Blood-Brain Barrier
Neoplasm Metastasis
Brain
Tight Junction Proteins
Permeability
Luciferases
Melanoma
Claudin-5
Occludin
Tight Junctions
Poisons
Vascular Endothelium
Internal Carotid Artery
Microvessels
Fluorescein
Fluorescence Microscopy
Oral Administration
Neoplasms
Western Blotting

Keywords

  • Blood-brain barrier
  • Brain metastasis
  • Polychlorinated biphenyls
  • Tight junctions

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Public Health, Environmental and Occupational Health

Cite this

Polychlorinated biphenyls disrupt blood-brain barrier integrity and promote brain metastasis formation. / Seelbach, Melissa; Chen, Lei; Powell, Anita; Choi, Yean Jung; Zhang, Bei; Hennig, Bernhard; Toborek, Michal J.

In: Environmental Health Perspectives, Vol. 118, No. 4, 01.04.2010, p. 479-484.

Research output: Contribution to journalArticle

Seelbach, Melissa ; Chen, Lei ; Powell, Anita ; Choi, Yean Jung ; Zhang, Bei ; Hennig, Bernhard ; Toborek, Michal J. / Polychlorinated biphenyls disrupt blood-brain barrier integrity and promote brain metastasis formation. In: Environmental Health Perspectives. 2010 ; Vol. 118, No. 4. pp. 479-484.
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