Polyamines in the anemia of end-stage renal disease

David Kushner, Barbara Beckman, Lyn Nguyen, Steven Chen, Christopher Della Santina, Fred Husserl, Janet Rice, James W. Fisher

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera from patients with ESRD inhibited erythroid colony (CFU-E) (N = 10) formation to a significantly (P < 0.011 greater degree than granulocyte-macrophage (CFU-GM) (N - 7) colony formation in mouse bone marrow (MBM) cultures. The polyamines spermine (SP) (18 to 560 nm/ml) and spermidine (SD) (4 to 648 nm/ml) exerted a more significant (P < 0.05) inhibition of CFU-E (N ≥ 5) than that of CFU-GM (N ≥ 5) growth. Concentrations of 0.80, 1.0, and 1.5 nm/ml of putrescine (PU) were 92%, 85%, and 77% of erythroid colony (CFU-E) controls (N = 4) and 104%, 130%, and 127% of CFU-GM controls (N = 4). Putrescine (PU) at 1.5 nm/ml also produced a significant (P < 0.05) inhibition of CFU-E, whereas CFU-GM were stimulated by PU. These data suggest that predialysis sera from uremic patients, as well as SP, SD, and PU, are selectively more inhibitory to CFU-E than CFU-GM growth. The immunoreactivity of EPO was not significantly changed when it was coincubated with SP, SD and PU and measured by radioimmunoassay. PU was found to inhibit noncompetitively the bioactivity of EPO in a CFU-E assay. These data support the hypothesis that polyamines may be important uremic toxins in the anemia of ESRD.

Original languageEnglish
Pages (from-to)725-732
Number of pages8
JournalKidney International
Volume39
Issue number4
StatePublished - Apr 1 1991
Externally publishedYes

Fingerprint

Erythroid Precursor Cells
Putrescine
Polyamines
Granulocyte-Macrophage Progenitor Cells
Chronic Kidney Failure
Anemia
Spermidine
Spermine
Erythropoietin
Serum
Continuous Ambulatory Peritoneal Dialysis
Erythropoiesis
Growth
Granulocytes
Radioimmunoassay
Bone Marrow
Macrophages

ASJC Scopus subject areas

  • Nephrology

Cite this

Kushner, D., Beckman, B., Nguyen, L., Chen, S., Della Santina, C., Husserl, F., ... Fisher, J. W. (1991). Polyamines in the anemia of end-stage renal disease. Kidney International, 39(4), 725-732.

Polyamines in the anemia of end-stage renal disease. / Kushner, David; Beckman, Barbara; Nguyen, Lyn; Chen, Steven; Della Santina, Christopher; Husserl, Fred; Rice, Janet; Fisher, James W.

In: Kidney International, Vol. 39, No. 4, 01.04.1991, p. 725-732.

Research output: Contribution to journalArticle

Kushner, D, Beckman, B, Nguyen, L, Chen, S, Della Santina, C, Husserl, F, Rice, J & Fisher, JW 1991, 'Polyamines in the anemia of end-stage renal disease', Kidney International, vol. 39, no. 4, pp. 725-732.
Kushner D, Beckman B, Nguyen L, Chen S, Della Santina C, Husserl F et al. Polyamines in the anemia of end-stage renal disease. Kidney International. 1991 Apr 1;39(4):725-732.
Kushner, David ; Beckman, Barbara ; Nguyen, Lyn ; Chen, Steven ; Della Santina, Christopher ; Husserl, Fred ; Rice, Janet ; Fisher, James W. / Polyamines in the anemia of end-stage renal disease. In: Kidney International. 1991 ; Vol. 39, No. 4. pp. 725-732.
@article{022b7ba8234d49adb9ceab94c41faee9,
title = "Polyamines in the anemia of end-stage renal disease",
abstract = "The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera from patients with ESRD inhibited erythroid colony (CFU-E) (N = 10) formation to a significantly (P < 0.011 greater degree than granulocyte-macrophage (CFU-GM) (N - 7) colony formation in mouse bone marrow (MBM) cultures. The polyamines spermine (SP) (18 to 560 nm/ml) and spermidine (SD) (4 to 648 nm/ml) exerted a more significant (P < 0.05) inhibition of CFU-E (N ≥ 5) than that of CFU-GM (N ≥ 5) growth. Concentrations of 0.80, 1.0, and 1.5 nm/ml of putrescine (PU) were 92{\%}, 85{\%}, and 77{\%} of erythroid colony (CFU-E) controls (N = 4) and 104{\%}, 130{\%}, and 127{\%} of CFU-GM controls (N = 4). Putrescine (PU) at 1.5 nm/ml also produced a significant (P < 0.05) inhibition of CFU-E, whereas CFU-GM were stimulated by PU. These data suggest that predialysis sera from uremic patients, as well as SP, SD, and PU, are selectively more inhibitory to CFU-E than CFU-GM growth. The immunoreactivity of EPO was not significantly changed when it was coincubated with SP, SD and PU and measured by radioimmunoassay. PU was found to inhibit noncompetitively the bioactivity of EPO in a CFU-E assay. These data support the hypothesis that polyamines may be important uremic toxins in the anemia of ESRD.",
author = "David Kushner and Barbara Beckman and Lyn Nguyen and Steven Chen and {Della Santina}, Christopher and Fred Husserl and Janet Rice and Fisher, {James W.}",
year = "1991",
month = "4",
day = "1",
language = "English",
volume = "39",
pages = "725--732",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Polyamines in the anemia of end-stage renal disease

AU - Kushner, David

AU - Beckman, Barbara

AU - Nguyen, Lyn

AU - Chen, Steven

AU - Della Santina, Christopher

AU - Husserl, Fred

AU - Rice, Janet

AU - Fisher, James W.

PY - 1991/4/1

Y1 - 1991/4/1

N2 - The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera from patients with ESRD inhibited erythroid colony (CFU-E) (N = 10) formation to a significantly (P < 0.011 greater degree than granulocyte-macrophage (CFU-GM) (N - 7) colony formation in mouse bone marrow (MBM) cultures. The polyamines spermine (SP) (18 to 560 nm/ml) and spermidine (SD) (4 to 648 nm/ml) exerted a more significant (P < 0.05) inhibition of CFU-E (N ≥ 5) than that of CFU-GM (N ≥ 5) growth. Concentrations of 0.80, 1.0, and 1.5 nm/ml of putrescine (PU) were 92%, 85%, and 77% of erythroid colony (CFU-E) controls (N = 4) and 104%, 130%, and 127% of CFU-GM controls (N = 4). Putrescine (PU) at 1.5 nm/ml also produced a significant (P < 0.05) inhibition of CFU-E, whereas CFU-GM were stimulated by PU. These data suggest that predialysis sera from uremic patients, as well as SP, SD, and PU, are selectively more inhibitory to CFU-E than CFU-GM growth. The immunoreactivity of EPO was not significantly changed when it was coincubated with SP, SD and PU and measured by radioimmunoassay. PU was found to inhibit noncompetitively the bioactivity of EPO in a CFU-E assay. These data support the hypothesis that polyamines may be important uremic toxins in the anemia of ESRD.

AB - The improvement in the anemia in patients with end-stage renal disease (ESRD) on continuous ambulatory peritoneal dialysis (CAPD) suggests that dialyzable substances present in the sera of uremic patients either inhibit erythropoiesis directly or inactivate erythropoietin (EPO). In the present study predialysis sera from patients with ESRD inhibited erythroid colony (CFU-E) (N = 10) formation to a significantly (P < 0.011 greater degree than granulocyte-macrophage (CFU-GM) (N - 7) colony formation in mouse bone marrow (MBM) cultures. The polyamines spermine (SP) (18 to 560 nm/ml) and spermidine (SD) (4 to 648 nm/ml) exerted a more significant (P < 0.05) inhibition of CFU-E (N ≥ 5) than that of CFU-GM (N ≥ 5) growth. Concentrations of 0.80, 1.0, and 1.5 nm/ml of putrescine (PU) were 92%, 85%, and 77% of erythroid colony (CFU-E) controls (N = 4) and 104%, 130%, and 127% of CFU-GM controls (N = 4). Putrescine (PU) at 1.5 nm/ml also produced a significant (P < 0.05) inhibition of CFU-E, whereas CFU-GM were stimulated by PU. These data suggest that predialysis sera from uremic patients, as well as SP, SD, and PU, are selectively more inhibitory to CFU-E than CFU-GM growth. The immunoreactivity of EPO was not significantly changed when it was coincubated with SP, SD and PU and measured by radioimmunoassay. PU was found to inhibit noncompetitively the bioactivity of EPO in a CFU-E assay. These data support the hypothesis that polyamines may be important uremic toxins in the anemia of ESRD.

UR - http://www.scopus.com/inward/record.url?scp=0025828932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025828932&partnerID=8YFLogxK

M3 - Article

C2 - 2051730

AN - SCOPUS:0025828932

VL - 39

SP - 725

EP - 732

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 4

ER -