Poly (4-vinylimidazole) as a non-viral gene delivery system

J. E. Ihm, K. O. Han, D. K. Han, K. D. Ahn, J. A. Hubbell, C. S. Cho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
StatePublished - Dec 1 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

Fingerprint

DNA
Genes
Spectrophotometers
Electrophoresis
Light scattering
Toxicity
Assays
Protons
Gels
Dyes
Particle size
Polymerization
Cells
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ihm, J. E., Han, K. O., Han, D. K., Ahn, K. D., Hubbell, J. A., & Cho, C. S. (2004). Poly (4-vinylimidazole) as a non-viral gene delivery system. In Transactions - 7th World Biomaterials Congress

Poly (4-vinylimidazole) as a non-viral gene delivery system. / Ihm, J. E.; Han, K. O.; Han, D. K.; Ahn, K. D.; Hubbell, J. A.; Cho, C. S.

Transactions - 7th World Biomaterials Congress. 2004.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ihm, JE, Han, KO, Han, DK, Ahn, KD, Hubbell, JA & Cho, CS 2004, Poly (4-vinylimidazole) as a non-viral gene delivery system. in Transactions - 7th World Biomaterials Congress. Transactions - 7th World Biomaterials Congress, Sydney, Australia, 5/17/04.
Ihm JE, Han KO, Han DK, Ahn KD, Hubbell JA, Cho CS. Poly (4-vinylimidazole) as a non-viral gene delivery system. In Transactions - 7th World Biomaterials Congress. 2004
Ihm, J. E. ; Han, K. O. ; Han, D. K. ; Ahn, K. D. ; Hubbell, J. A. ; Cho, C. S. / Poly (4-vinylimidazole) as a non-viral gene delivery system. Transactions - 7th World Biomaterials Congress. 2004.
@inproceedings{f8c75cd7e63b4661a7ce93bc48631e15,
title = "Poly (4-vinylimidazole) as a non-viral gene delivery system",
abstract = "The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.",
author = "Ihm, {J. E.} and Han, {K. O.} and Han, {D. K.} and Ahn, {K. D.} and Hubbell, {J. A.} and Cho, {C. S.}",
year = "2004",
month = "12",
day = "1",
language = "English",
isbn = "1877040193",
booktitle = "Transactions - 7th World Biomaterials Congress",

}

TY - GEN

T1 - Poly (4-vinylimidazole) as a non-viral gene delivery system

AU - Ihm, J. E.

AU - Han, K. O.

AU - Han, D. K.

AU - Ahn, K. D.

AU - Hubbell, J. A.

AU - Cho, C. S.

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.

AB - The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.

UR - http://www.scopus.com/inward/record.url?scp=13844271526&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844271526&partnerID=8YFLogxK

M3 - Conference contribution

SN - 1877040193

SN - 9781877040191

BT - Transactions - 7th World Biomaterials Congress

ER -