Abstract
The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.
| Original language | English (US) |
|---|---|
| Title of host publication | Transactions - 7th World Biomaterials Congress |
| Number of pages | 1 |
| State | Published - Dec 1 2004 |
| Event | Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: May 17 2004 → May 21 2004 |
Publication series
| Name | Transactions - 7th World Biomaterials Congress |
|---|
Other
| Other | Transactions - 7th World Biomaterials Congress |
|---|---|
| Country | Australia |
| City | Sydney |
| Period | 5/17/04 → 5/21/04 |
ASJC Scopus subject areas
- Engineering(all)
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Cite this
Ihm, J. E., Han, K. O., Han, D. K., Ahn, K. D., Hubbell, J. A., & Cho, C. S. (2004). Poly (4-vinylimidazole) as a non-viral gene delivery system. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).