Poly (4-vinylimidazole) as a non-viral gene delivery system

J. E. Ihm, K. O. Han, D. K. Han, K. D. Ahn, J. A. Hubbell, C. S. Cho

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.

Original languageEnglish (US)
Title of host publicationTransactions - 7th World Biomaterials Congress
Pages1811
Number of pages1
StatePublished - 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
Country/TerritoryAustralia
CitySydney
Period5/17/045/21/04

ASJC Scopus subject areas

  • Engineering(all)

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