Abstract
The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.
| Original language | English |
|---|---|
| Title of host publication | Transactions - 7th World Biomaterials Congress |
| State | Published - Dec 1 2004 |
| Externally published | Yes |
| Event | Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: May 17 2004 → May 21 2004 |
Other
| Other | Transactions - 7th World Biomaterials Congress |
|---|---|
| Country | Australia |
| City | Sydney |
| Period | 5/17/04 → 5/21/04 |
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ASJC Scopus subject areas
- Engineering(all)
Cite this
Poly (4-vinylimidazole) as a non-viral gene delivery system. / Ihm, J. E.; Han, K. O.; Han, D. K.; Ahn, K. D.; Hubbell, J. A.; Cho, C. S.
Transactions - 7th World Biomaterials Congress. 2004.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Poly (4-vinylimidazole) as a non-viral gene delivery system
AU - Ihm, J. E.
AU - Han, K. O.
AU - Han, D. K.
AU - Ahn, K. D.
AU - Hubbell, J. A.
AU - Cho, C. S.
PY - 2004/12/1
Y1 - 2004/12/1
N2 - The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.
AB - The possibility of a gene carrier was investigated by in vitro transfection experiment with several cell lines. The poly(4-vinyl imidazole)(P4V) was synthesized by polymerization of 4-vinylimidazole (4V). The complex formation between P4V and DNA was confirmed by agarose gel electrophoresis and dye-exclusion assay. Using a light scattering spectrophotometer, particle sizes were determined. It was shown that The P4V/DNA complex exhibited very high transfection efficiency through the proton sponge mechanism of the imidazole groups with low cell toxicity.
UR - http://www.scopus.com/inward/record.url?scp=13844271526&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844271526&partnerID=8YFLogxK
M3 - Conference contribution
SN - 1877040193
SN - 9781877040191
BT - Transactions - 7th World Biomaterials Congress
ER -