POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS

Danuta Frackowiak, Elisabeth Gantt, Surat Hotchandani, Claudia A. Lipschultz, Roger Leblanc

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Abstract— Phycobilisomes from Porphyridium cruentum, suspended in polyvinyl alcohol were found to be highly stable, and had normal absorption and fluorescence spectra. Intact phycobilisomes had a major emission peak at 680 nm, whereas upon partial dissociating the major emission was at 580 nm. Incorporation of phycobilisomes in thin polyvinyl alcohol films facilitated examination by photoacoustic spectroscopy. The photoacoustic spectra had a broad absorption maximum at 545–565 nm (phycoerythrin), which resolved as two peaks (545 and 563 nm) in absorption spectra. Stretching of films resulted in apparent chromophore reorientation in partially dissociated, but not in intact phycobilisomes. Only in dissociated phycobilisomes was observed a differential chromophore orientation at 685 nm by polarized fluorescence, which is attributed to a change in orientation of the terminal phycobilisome pigment relative to phycoerythrin.

Original languageEnglish (US)
Pages (from-to)335-337
Number of pages3
JournalPhotochemistry and Photobiology
Volume43
Issue number3
DOIs
StatePublished - 1986
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

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