POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS

Danuta Frackowiak; Elisabeth Gantt; Surat Hotchandani; Claudia A. Lipschultz; Roger Leblanc

doi:10.1111/j.1751-1097.1986.tb05613.x

POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS

Danuta Frackowiak, Elisabeth Gantt, Surat Hotchandani, Claudia A. Lipschultz, Roger Leblanc

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Abstract— Phycobilisomes from Porphyridium cruentum, suspended in polyvinyl alcohol were found to be highly stable, and had normal absorption and fluorescence spectra. Intact phycobilisomes had a major emission peak at 680 nm, whereas upon partial dissociating the major emission was at 580 nm. Incorporation of phycobilisomes in thin polyvinyl alcohol films facilitated examination by photoacoustic spectroscopy. The photoacoustic spectra had a broad absorption maximum at 545–565 nm (phycoerythrin), which resolved as two peaks (545 and 563 nm) in absorption spectra. Stretching of films resulted in apparent chromophore reorientation in partially dissociated, but not in intact phycobilisomes. Only in dissociated phycobilisomes was observed a differential chromophore orientation at 685 nm by polarized fluorescence, which is attributed to a change in orientation of the terminal phycobilisome pigment relative to phycoerythrin.

Original language	English (US)
Pages (from-to)	335-337
Number of pages	3
Journal	Photochemistry and Photobiology
Volume	43
Issue number	3
DOIs	https://doi.org/10.1111/j.1751-1097.1986.tb05613.x
State	Published - 1986
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)
Biochemistry
Physical and Theoretical Chemistry

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10.1111/j.1751-1097.1986.tb05613.x

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title = "POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS",

abstract = "Abstract— Phycobilisomes from Porphyridium cruentum, suspended in polyvinyl alcohol were found to be highly stable, and had normal absorption and fluorescence spectra. Intact phycobilisomes had a major emission peak at 680 nm, whereas upon partial dissociating the major emission was at 580 nm. Incorporation of phycobilisomes in thin polyvinyl alcohol films facilitated examination by photoacoustic spectroscopy. The photoacoustic spectra had a broad absorption maximum at 545–565 nm (phycoerythrin), which resolved as two peaks (545 and 563 nm) in absorption spectra. Stretching of films resulted in apparent chromophore reorientation in partially dissociated, but not in intact phycobilisomes. Only in dissociated phycobilisomes was observed a differential chromophore orientation at 685 nm by polarized fluorescence, which is attributed to a change in orientation of the terminal phycobilisome pigment relative to phycoerythrin.",

author = "Danuta Frackowiak and Elisabeth Gantt and Surat Hotchandani and Lipschultz, {Claudia A.} and Roger Leblanc",

year = "1986",

doi = "10.1111/j.1751-1097.1986.tb05613.x",

language = "English (US)",

volume = "43",

pages = "335--337",

journal = "Photochemistry and Photobiology",

issn = "0031-8655",

publisher = "Wiley-Blackwell",

number = "3",

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TY - JOUR

T1 - POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS

AU - Frackowiak, Danuta

AU - Gantt, Elisabeth

AU - Hotchandani, Surat

AU - Lipschultz, Claudia A.

AU - Leblanc, Roger

PY - 1986

Y1 - 1986

N2 - Abstract— Phycobilisomes from Porphyridium cruentum, suspended in polyvinyl alcohol were found to be highly stable, and had normal absorption and fluorescence spectra. Intact phycobilisomes had a major emission peak at 680 nm, whereas upon partial dissociating the major emission was at 580 nm. Incorporation of phycobilisomes in thin polyvinyl alcohol films facilitated examination by photoacoustic spectroscopy. The photoacoustic spectra had a broad absorption maximum at 545–565 nm (phycoerythrin), which resolved as two peaks (545 and 563 nm) in absorption spectra. Stretching of films resulted in apparent chromophore reorientation in partially dissociated, but not in intact phycobilisomes. Only in dissociated phycobilisomes was observed a differential chromophore orientation at 685 nm by polarized fluorescence, which is attributed to a change in orientation of the terminal phycobilisome pigment relative to phycoerythrin.

AB - Abstract— Phycobilisomes from Porphyridium cruentum, suspended in polyvinyl alcohol were found to be highly stable, and had normal absorption and fluorescence spectra. Intact phycobilisomes had a major emission peak at 680 nm, whereas upon partial dissociating the major emission was at 580 nm. Incorporation of phycobilisomes in thin polyvinyl alcohol films facilitated examination by photoacoustic spectroscopy. The photoacoustic spectra had a broad absorption maximum at 545–565 nm (phycoerythrin), which resolved as two peaks (545 and 563 nm) in absorption spectra. Stretching of films resulted in apparent chromophore reorientation in partially dissociated, but not in intact phycobilisomes. Only in dissociated phycobilisomes was observed a differential chromophore orientation at 685 nm by polarized fluorescence, which is attributed to a change in orientation of the terminal phycobilisome pigment relative to phycoerythrin.

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JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 3

ER -

POLARIZED PHOTO ACOUSTIC SPECTRA OF PHYCOBILISOMES IN POLYVINYL ALCOHOL FILMS

Abstract

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