Platelets activated during myocardial infarction release functional miRNA, which can be taken up by endothelial cells and regulate ICAM1 expression.

Olof Gidlöf, Marcel van der Brug, Jenny Ohman, Patrik Gilje, Björn Olde, Claes R Wahlestedt, David Erlinge

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Platelets play a crucial role in the pathogenesis of myocardial infarction (MI) by adhering to the site of a ruptured atherosclerotic plaque. The aim of this study was to screen for differences in the micro RNA (miRNA) content of platelets from patients with myocardial infarction and control patients, to investigate a possible release of miRNAs from activated platelets and to elucidate whether platelet-derived miRNAs could act as paracrine regulators of endothelial cell gene expression. Using RNA-seq, we found 9 differentially expressed miRNAs in patients compared with healthy controls, of which 8 were decreased in patients. Of these, miR-22, -185, -320b, and -423-5p increased in the supernatant of platelets after aggregation and were depleted in thrombi aspirated from MI patients, indicating the release of certain miRNAs from activated platelets. To confirm that endothelial cells could take up the released platelet miRNAs, transfer of both fluorescently labeled miRNA and exogenous cel-miR-39 from activated platelets to endothelial cells was shown. Finally, a possible paracrine role of released platelet miR-320b on endothelial cell intercellular adhesion molecule-1 expression was shown. Thus, platelets from patients with MI exhibit loss of specific miRNAs, and activated platelets shed miRNAs that can regulate endothelial cell gene expression.

Original languageEnglish
JournalBlood
Volume121
Issue number19
DOIs
StatePublished - May 9 2013

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Endothelial cells
Platelets
MicroRNAs
Blood Platelets
Endothelial Cells
Myocardial Infarction
Gene expression
Gene Expression
Cell Adhesion Molecules
Atherosclerotic Plaques
Intercellular Adhesion Molecule-1
Platelet Aggregation
Thrombosis
Agglomeration
RNA

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Platelets activated during myocardial infarction release functional miRNA, which can be taken up by endothelial cells and regulate ICAM1 expression. / Gidlöf, Olof; van der Brug, Marcel; Ohman, Jenny; Gilje, Patrik; Olde, Björn; Wahlestedt, Claes R; Erlinge, David.

In: Blood, Vol. 121, No. 19, 09.05.2013.

Research output: Contribution to journalArticle

Gidlöf, Olof ; van der Brug, Marcel ; Ohman, Jenny ; Gilje, Patrik ; Olde, Björn ; Wahlestedt, Claes R ; Erlinge, David. / Platelets activated during myocardial infarction release functional miRNA, which can be taken up by endothelial cells and regulate ICAM1 expression. In: Blood. 2013 ; Vol. 121, No. 19.
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