Platelet-activating factor modulates a secreted phosphatase activity of the trypanosomatid parasite Herpetomonas muscarum muscarum

Patricia M.L. Dutra, Felipe A. Dias, Claudia O. Rodrigues, Alexandre Romeiro, Marcia Attias, Wanderley De Souza, Angela H.C.S. Lopes, José Roberto Meyer-Fernandes

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

In the present work we characterized the secreted phosphatase activity of the trypanosomatid parasite Herpetomonas muscarum muscarum. This housefly parasite hydrolyzed p-nitrophenylphosphate at a rate of 10.26 nmol Pi/mg protein/min. Classical inhibitors of acid phosphatases, such as sodium orthovanadate (NaVO3), sodium fluoride (NaF), and ammonium molybdate promoted a decrease in this phosphatase activity. When the parasites were assayed in the presence of sodium tartrate, an inhibitor of Leishmania spp-secreted acid phosphatases, this activity was drastically diminished. Cytochemical analysis showed the localization of this enzyme on the external surface and in the flagellar pocket of these parasites. Sodium tartrate inhibited this reaction, confirming the biochemical data. Platelet-activating factor (PAF) inhibited the phosphatase activity determined in the supernatant of living H. m. muscarum.

Original languageEnglish (US)
Pages (from-to)288-292
Number of pages5
JournalCurrent Microbiology
Volume43
Issue number4
DOIs
StatePublished - Sep 12 2001
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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