Abstract
Study objective: In search of a better universal marker of tissue inflammation in allergic reactions, we studied platelet activation and its relation to allergic asthma in sheep. Setting: University research laboratory. Subjects: Twenty-three allergic sheep. Design and interventions: We serially measured plasma thrombospondin (TSP), a glycoprotein secreted from activated platelets, using enzyme-linked immunosorbent assay with a monoclonal antibody. We validated the assay by establishing a standard curve that closely matches that of humans. Measurements and results: Of the 23 allergic sheep tested, 10 demonstrated early airway response (ER group) and 13 had early and late dual airway responses (DR group) to inhaled Ascaris suum antigen. TSP was measured before, and at 0, 1, 2, 3, 4, 6, 8, and 24 h postantigen challenge. Both groups showed an increase in plasma TSP level after antigen challenge, but only the DR group showed a significant increase in TSP at 1 h (threefold) and 8 h (sixfold) postchallenge. The two sheep that received carbachol challenge showed a slight rise in TSP at 8 h postchallenge. There was a significant correlation between the TSP level at 1 h and the peak early airway response in the DR group. Conclusions: These results suggest that the two groups of allergic sheep may have two distinct induction processes leading to airway obstruction. The dual peak rise in TSP level that coincided with the airway response also suggests that an early priming effect exists in the DR group, which might have led to the late response. We conclude that intravascular platelet activation often occurs in mast cell-mediated allergic disorders. Therefore, plasma TSP may be useful for monitoring the course of allergic disorders.
| Original language | English |
|---|---|
| Pages (from-to) | 1614-1617 |
| Number of pages | 4 |
| Journal | Chest |
| Volume | 109 |
| Issue number | 6 |
| State | Published - Jun 25 1996 |
| Externally published | Yes |
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Keywords
- allergy
- blood
- plasma
- platelets
- thrombospondin
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
Cite this
Plasma thrombospondin levels in sheep with allergic asthma. / Huang, Shih Wen; Kao, Kuo Jung; Abraham, William M.
In: Chest, Vol. 109, No. 6, 25.06.1996, p. 1614-1617.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasma thrombospondin levels in sheep with allergic asthma
AU - Huang, Shih Wen
AU - Kao, Kuo Jung
AU - Abraham, William M.
PY - 1996/6/25
Y1 - 1996/6/25
N2 - Study objective: In search of a better universal marker of tissue inflammation in allergic reactions, we studied platelet activation and its relation to allergic asthma in sheep. Setting: University research laboratory. Subjects: Twenty-three allergic sheep. Design and interventions: We serially measured plasma thrombospondin (TSP), a glycoprotein secreted from activated platelets, using enzyme-linked immunosorbent assay with a monoclonal antibody. We validated the assay by establishing a standard curve that closely matches that of humans. Measurements and results: Of the 23 allergic sheep tested, 10 demonstrated early airway response (ER group) and 13 had early and late dual airway responses (DR group) to inhaled Ascaris suum antigen. TSP was measured before, and at 0, 1, 2, 3, 4, 6, 8, and 24 h postantigen challenge. Both groups showed an increase in plasma TSP level after antigen challenge, but only the DR group showed a significant increase in TSP at 1 h (threefold) and 8 h (sixfold) postchallenge. The two sheep that received carbachol challenge showed a slight rise in TSP at 8 h postchallenge. There was a significant correlation between the TSP level at 1 h and the peak early airway response in the DR group. Conclusions: These results suggest that the two groups of allergic sheep may have two distinct induction processes leading to airway obstruction. The dual peak rise in TSP level that coincided with the airway response also suggests that an early priming effect exists in the DR group, which might have led to the late response. We conclude that intravascular platelet activation often occurs in mast cell-mediated allergic disorders. Therefore, plasma TSP may be useful for monitoring the course of allergic disorders.
AB - Study objective: In search of a better universal marker of tissue inflammation in allergic reactions, we studied platelet activation and its relation to allergic asthma in sheep. Setting: University research laboratory. Subjects: Twenty-three allergic sheep. Design and interventions: We serially measured plasma thrombospondin (TSP), a glycoprotein secreted from activated platelets, using enzyme-linked immunosorbent assay with a monoclonal antibody. We validated the assay by establishing a standard curve that closely matches that of humans. Measurements and results: Of the 23 allergic sheep tested, 10 demonstrated early airway response (ER group) and 13 had early and late dual airway responses (DR group) to inhaled Ascaris suum antigen. TSP was measured before, and at 0, 1, 2, 3, 4, 6, 8, and 24 h postantigen challenge. Both groups showed an increase in plasma TSP level after antigen challenge, but only the DR group showed a significant increase in TSP at 1 h (threefold) and 8 h (sixfold) postchallenge. The two sheep that received carbachol challenge showed a slight rise in TSP at 8 h postchallenge. There was a significant correlation between the TSP level at 1 h and the peak early airway response in the DR group. Conclusions: These results suggest that the two groups of allergic sheep may have two distinct induction processes leading to airway obstruction. The dual peak rise in TSP level that coincided with the airway response also suggests that an early priming effect exists in the DR group, which might have led to the late response. We conclude that intravascular platelet activation often occurs in mast cell-mediated allergic disorders. Therefore, plasma TSP may be useful for monitoring the course of allergic disorders.
KW - allergy
KW - blood
KW - plasma
KW - platelets
KW - thrombospondin
UR - http://www.scopus.com/inward/record.url?scp=0029941975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029941975&partnerID=8YFLogxK
M3 - Article
C2 - 8769519
AN - SCOPUS:0029941975
VL - 109
SP - 1614
EP - 1617
JO - Chest
JF - Chest
SN - 0012-3692
IS - 6
ER -