Plasma protein electrophoresis and select acute phase proteins in healthy bonnethead sharks (Sphyrna tiburo) under managed care

Michael W. Hyatt, Cara L. Field, Tonya M. Clauss, Kristopher Arheart, Carolyn Cray

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Preventative health care of elasmobranchs is an important but understudied field of aquatic veterinary medicine. Evaluation of inflammation through the acute phase response is a valuable tool in health assessments. To better assess the health of bonnethead sharks (Sphyrna tiburo) under managed care, normal reference intervals of protein electrophoresis (EPH) and the acute phase proteins, C-reactive protein (CRP) and haptoglobin (HP), were established. Blood was collected from wild caught, captive raised bonnethead sharks housed at public aquaria. Lithium heparinized plasma was either submitted fresh or stored at -80°C prior to submission. Electrophoresis identified protein fractions with migration characteristics similar to other animals with albumin, α-1 globulin, α-2 globulin, β globulin, and γ globulin. These fractions were classified as fractions 1-5 as fractional contents are unknown in this species. Commercial reagents for CRP and HP were validated for use in bonnethead sharks. Reference intervals were established using the robust method recommended by the American Society for Veterinary Clinical Pathology for the calculation of 90% reference intervals. Once established, the diagnostic and clinical applicability of these reference intervals was used to assess blood from individuals with known infectious diseases that resulted in systemic inflammation and eventual death. Unhealthy bonnethead sharks had significantly decreased fraction 2, fraction 3, and fraction 3:4 ratio and significantly increased fraction 5, CRP, and HP. These findings advance our understanding of elasmobranch acute phase inflammatory response and health and aid clinicians in the diagnosis of inflammatory disease in bonnethead sharks.

Original languageEnglish (US)
Pages (from-to)984-992
Number of pages9
JournalJournal of Zoo and Wildlife Medicine
Volume47
Issue number4
DOIs
StatePublished - Dec 1 2016

Fingerprint

Sphyrna
Sharks
acute phase proteins
Acute-Phase Proteins
Managed Care Programs
sharks
blood proteins
electrophoresis
Globulins
Electrophoresis
Blood Proteins
globulins
Haptoglobins
haptoglobins
C-reactive protein
C-Reactive Protein
Elasmobranchii
Acute-Phase Reaction
inflammation
Health

Keywords

  • Acute phase
  • C-reactive protein
  • Electrophoresis
  • Haptoglobin
  • Reference interval
  • Shark

ASJC Scopus subject areas

  • Medicine(all)
  • Animal Science and Zoology
  • veterinary(all)

Cite this

Plasma protein electrophoresis and select acute phase proteins in healthy bonnethead sharks (Sphyrna tiburo) under managed care. / Hyatt, Michael W.; Field, Cara L.; Clauss, Tonya M.; Arheart, Kristopher; Cray, Carolyn.

In: Journal of Zoo and Wildlife Medicine, Vol. 47, No. 4, 01.12.2016, p. 984-992.

Research output: Contribution to journalArticle

Hyatt, Michael W. ; Field, Cara L. ; Clauss, Tonya M. ; Arheart, Kristopher ; Cray, Carolyn. / Plasma protein electrophoresis and select acute phase proteins in healthy bonnethead sharks (Sphyrna tiburo) under managed care. In: Journal of Zoo and Wildlife Medicine. 2016 ; Vol. 47, No. 4. pp. 984-992.
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