Plasma protein electrophoresis and acute phase proteins in koi carp (Cyprinus carpio) following exploratory coeliotomy

Emily F. Christiansen, Carolyn Cray, Gregory A. Lewbart, Craig A. Harms

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Measuring plasma protein electrophoresis fractions and plasma concentrations of acute phase proteins (APPs) is commonly used to monitor inflammation and illness in mammalian species, but its use is not widespread in fish species, despite the presence of similar proteins. This study aims to determine plasma protein electrophoresis values for koi carp (Cyprinus carpio) and to assess the use of plasma protein electrophoresis and commercially available assays for 3 primary APPs (C-reactive protein, serum amyloid A, and haptoglobin) as markers of inflammation in 13 koi following invasive surgery. Median packed cell volume decreased by nearly 50% but recovered to baseline by 14 days postsurgery. Plasma protein electrophoresis fractions were consistent across the experimental period and did not demonstrate an inflammatory pattern, but electrophoretograms exhibited variation between individual fish. No clinically meaningful changes were observed in the APPs of the test subjects 28 days following surgery. Despite grossly evident inflammation of the incision line, either this surgical procedure did not stimulate a marked systemic inflammatory response, or the available assays are not effective in detecting the acute phase inflammatory response in this fish species.

Original languageEnglish
Pages (from-to)76-83
Number of pages8
JournalJournal of Exotic Pet Medicine
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

koi
acute phase proteins
Carps
Acute-Phase Proteins
Cyprinus carpio
carp
blood proteins
electrophoresis
Electrophoresis
Blood Proteins
inflammation
Fishes
Inflammation
surgery
Serum Amyloid A Protein
Acute-Phase Reaction
Haptoglobins
fish
Protein C
Ambulatory Surgical Procedures

Keywords

  • Acute phase response
  • Koi carp
  • Protein electrophoresis
  • Surgery

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Plasma protein electrophoresis and acute phase proteins in koi carp (Cyprinus carpio) following exploratory coeliotomy. / Christiansen, Emily F.; Cray, Carolyn; Lewbart, Gregory A.; Harms, Craig A.

In: Journal of Exotic Pet Medicine, Vol. 24, No. 1, 01.01.2015, p. 76-83.

Research output: Contribution to journalArticle

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