Abstract
Measuring plasma protein electrophoresis fractions and plasma concentrations of acute phase proteins (APPs) is commonly used to monitor inflammation and illness in mammalian species, but its use is not widespread in fish species, despite the presence of similar proteins. This study aims to determine plasma protein electrophoresis values for koi carp (Cyprinus carpio) and to assess the use of plasma protein electrophoresis and commercially available assays for 3 primary APPs (C-reactive protein, serum amyloid A, and haptoglobin) as markers of inflammation in 13 koi following invasive surgery. Median packed cell volume decreased by nearly 50% but recovered to baseline by 14 days postsurgery. Plasma protein electrophoresis fractions were consistent across the experimental period and did not demonstrate an inflammatory pattern, but electrophoretograms exhibited variation between individual fish. No clinically meaningful changes were observed in the APPs of the test subjects 28 days following surgery. Despite grossly evident inflammation of the incision line, either this surgical procedure did not stimulate a marked systemic inflammatory response, or the available assays are not effective in detecting the acute phase inflammatory response in this fish species.
| Original language | English |
|---|---|
| Pages (from-to) | 76-83 |
| Number of pages | 8 |
| Journal | Journal of Exotic Pet Medicine |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2015 |
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Keywords
- Acute phase response
- Koi carp
- Protein electrophoresis
- Surgery
ASJC Scopus subject areas
- veterinary(all)
Cite this
Plasma protein electrophoresis and acute phase proteins in koi carp (Cyprinus carpio) following exploratory coeliotomy. / Christiansen, Emily F.; Cray, Carolyn; Lewbart, Gregory A.; Harms, Craig A.
In: Journal of Exotic Pet Medicine, Vol. 24, No. 1, 01.01.2015, p. 76-83.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasma protein electrophoresis and acute phase proteins in koi carp (Cyprinus carpio) following exploratory coeliotomy
AU - Christiansen, Emily F.
AU - Cray, Carolyn
AU - Lewbart, Gregory A.
AU - Harms, Craig A.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Measuring plasma protein electrophoresis fractions and plasma concentrations of acute phase proteins (APPs) is commonly used to monitor inflammation and illness in mammalian species, but its use is not widespread in fish species, despite the presence of similar proteins. This study aims to determine plasma protein electrophoresis values for koi carp (Cyprinus carpio) and to assess the use of plasma protein electrophoresis and commercially available assays for 3 primary APPs (C-reactive protein, serum amyloid A, and haptoglobin) as markers of inflammation in 13 koi following invasive surgery. Median packed cell volume decreased by nearly 50% but recovered to baseline by 14 days postsurgery. Plasma protein electrophoresis fractions were consistent across the experimental period and did not demonstrate an inflammatory pattern, but electrophoretograms exhibited variation between individual fish. No clinically meaningful changes were observed in the APPs of the test subjects 28 days following surgery. Despite grossly evident inflammation of the incision line, either this surgical procedure did not stimulate a marked systemic inflammatory response, or the available assays are not effective in detecting the acute phase inflammatory response in this fish species.
AB - Measuring plasma protein electrophoresis fractions and plasma concentrations of acute phase proteins (APPs) is commonly used to monitor inflammation and illness in mammalian species, but its use is not widespread in fish species, despite the presence of similar proteins. This study aims to determine plasma protein electrophoresis values for koi carp (Cyprinus carpio) and to assess the use of plasma protein electrophoresis and commercially available assays for 3 primary APPs (C-reactive protein, serum amyloid A, and haptoglobin) as markers of inflammation in 13 koi following invasive surgery. Median packed cell volume decreased by nearly 50% but recovered to baseline by 14 days postsurgery. Plasma protein electrophoresis fractions were consistent across the experimental period and did not demonstrate an inflammatory pattern, but electrophoretograms exhibited variation between individual fish. No clinically meaningful changes were observed in the APPs of the test subjects 28 days following surgery. Despite grossly evident inflammation of the incision line, either this surgical procedure did not stimulate a marked systemic inflammatory response, or the available assays are not effective in detecting the acute phase inflammatory response in this fish species.
KW - Acute phase response
KW - Koi carp
KW - Protein electrophoresis
KW - Surgery
UR - http://www.scopus.com/inward/record.url?scp=84920895908&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84920895908&partnerID=8YFLogxK
U2 - 10.1053/j.jepm.2014.11.008
DO - 10.1053/j.jepm.2014.11.008
M3 - Article
AN - SCOPUS:84920895908
VL - 24
SP - 76
EP - 83
JO - Journal of Exotic Pet Medicine
JF - Journal of Exotic Pet Medicine
SN - 1557-5063
IS - 1
ER -