Plasma metabolomic profile in fibrosing pulmonary sarcoidosis

Mehdi Mirsaeidi, Mohammad Mehdi Banoei, Caleb K. Nienow, Taimur Abassi, Anoushirvan Hakim, Dean Schraufnagel, Brent W. Winston, Nadera Sweiss, Robert Baughman, Joe G N Garcia, Roberto Machado

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: There is no known marker to screen patients with sarcoidosis to determine the risk of progression to pulmonary fibrosis. We aimed to identify potential noninvasive biomarkers for early detection of pulmonary fibrosing sarcoidosis. Methods: A case-control study was performed on African Americans with confirmed sarcoidosis included 31 subjects with pulmonary fibrosis vs. 36 without pulmonary fibrosis. Plasma samples were analyzed by liquid chromatography-mass spectrum. Multivariate statistical analysis models were developed in a training set based on 50 age- And sex-matched samples to identify metabolites involved in the discrimination. Principal component analysis and orthogonal partial least squares-discriminant (OPLS) analysis coupled to the most influential variables were used to derive significant metabolic discriminations. Results: Of the datasets from 171 feature peaks, 14 features including p-coumaroylagmatine and palmitoylcarnitine showed significant differences between fibrosing and non-fibrosing pulmonary sarcoidosis (p = 0.001). OPLS analysis presented clear separation between two groups with an acceptable goodness of fit (R2 = 0.522) and predictive power (Q2=0.322). Discriminating metabolites involved collagen pathway metabolites especially those in the arginine-proline pathway. Conclusions: Metabolomics can provide a useful tool to detect pulmonary fibrosis in patients with sarcoidosis. Two discriminating metabolites, p-coumaroylagmatine and palmitoylcarnitine may be potential markers for fibrosing pulmonary sarcoidosis. (Sarcoidosis Vasc Diffuse Lung Dis 2016; 33: 29-38).

Original languageEnglish (US)
Pages (from-to)29-38
Number of pages10
JournalSarcoidosis Vasculitis and Diffuse Lung Diseases
Volume33
Issue number1
StatePublished - 2016
Externally publishedYes

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Keywords

  • Fibrosis
  • Metabolomics
  • Sarcoidosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Immunology and Allergy
  • Internal Medicine

Cite this

Mirsaeidi, M., Banoei, M. M., Nienow, C. K., Abassi, T., Hakim, A., Schraufnagel, D., Winston, B. W., Sweiss, N., Baughman, R., Garcia, J. G. N., & Machado, R. (2016). Plasma metabolomic profile in fibrosing pulmonary sarcoidosis. Sarcoidosis Vasculitis and Diffuse Lung Diseases, 33(1), 29-38.