Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes

Marta Garcia-Contreras, Sanket H. Shah, Alejandro Tamayo, Paul D. Robbins, Ronald B. Golberg, Armando J Mendez, Camillo Ricordi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specific diagnostic signature. To assess the value of exosome miRNAs as biomarkers for T1DM, miRNA expression in plasma-derived exosomes was measured. Nanoparticle tracking analysis and transmission electron microscopy confirmed the presence of plasma-derived exosomes (EXOs) isolated by differential centrifugation. Total RNA extracted from plasma-derived EXOs of 12 T1DM and 12 control subjects was hybridized onto Nanostring human v2 miRNA microarray array and expression data were analyzed on nSolver analysis software. We found 7 different miRNAs (1 up-regulated and 6 down-regulated), that were differentially expressed in T1DM. The selected candidate miRNAs were validated by qRT-PCR analysis of cohorts of 24 T1DM and 24 control subjects. Most of the deregulated miRNAs are involved in progression of T1DM. These findings highlight the potential of EXOs miRNA profiling in the diagnosis as well as new insights into the molecular mechanisms involved in T1DM.

Original languageEnglish (US)
Article number5998
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Exosomes
MicroRNAs
Type 1 Diabetes Mellitus
Multivesicular Bodies
Transmission Electron Microscopy
Centrifugation
Hyperglycemia
Nanoparticles
Cohort Studies
Software
Biomarkers
RNA
Insulin
Lipids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • General

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Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes. / Garcia-Contreras, Marta; Shah, Sanket H.; Tamayo, Alejandro; Robbins, Paul D.; Golberg, Ronald B.; Mendez, Armando J; Ricordi, Camillo.

In: Scientific Reports, Vol. 7, No. 1, 5998, 01.12.2017.

Research output: Contribution to journalArticle

Garcia-Contreras, Marta ; Shah, Sanket H. ; Tamayo, Alejandro ; Robbins, Paul D. ; Golberg, Ronald B. ; Mendez, Armando J ; Ricordi, Camillo. / Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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