Plant Rac proteins induce superoxide production in mammalian cells

Hamdy H. Hassanain, Yogesh K. Sharma, Leni Moldovan, Valery Khramtsov, Lawrence J. Berliner, Jon P. Duvick, Pascal Goldschmidt-Clermont

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The small GTP-binding protein family including Rac proteins represents a paradigm for signaling molecules shared by animal and plants. In mammalian cells, Rac induces the activation of NADPH oxidase leading to superoxide production. In plants, evidence suggests that resistance to pathogens depends on superoxide that is generated via NADPH oxidase-like enzymes. We have identified four closely related Rho/Rac genes from Zea mays that exhibit a high degree of homology to the human Rac. We hypothesized that these plant Rac proteins could function as their mammalian counterpart and activate an enzymatic complex that leads to superoxide production. Here, we show that like human Rac1, activated Zea mays Rac genes can induce superoxide production, when expressed in a mammalian system: NIH 3T3 cells. Our results suggest that in plants, Rac proteins can function as activators of oxidative burst and indicate the remarkable functional and structural conservation of Rho/Rac proteins between plant and animal kingdoms during evolution. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)783-788
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume272
Issue number3
DOIs
StatePublished - Jun 16 2000
Externally publishedYes

Fingerprint

rac GTP-Binding Proteins
Plant Proteins
Superoxides
Cells
NADPH Oxidase
Zea mays
Animals
Genes
Activator Appliances
NIH 3T3 Cells
Respiratory Burst
Pathogens
GTP-Binding Proteins
Conservation
Chemical activation
Molecules
Enzymes

Keywords

  • Plant
  • Rac
  • Signal transduction
  • Superoxide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hassanain, H. H., Sharma, Y. K., Moldovan, L., Khramtsov, V., Berliner, L. J., Duvick, J. P., & Goldschmidt-Clermont, P. (2000). Plant Rac proteins induce superoxide production in mammalian cells. Biochemical and Biophysical Research Communications, 272(3), 783-788. https://doi.org/10.1006/bbrc.2000.2791

Plant Rac proteins induce superoxide production in mammalian cells. / Hassanain, Hamdy H.; Sharma, Yogesh K.; Moldovan, Leni; Khramtsov, Valery; Berliner, Lawrence J.; Duvick, Jon P.; Goldschmidt-Clermont, Pascal.

In: Biochemical and Biophysical Research Communications, Vol. 272, No. 3, 16.06.2000, p. 783-788.

Research output: Contribution to journalArticle

Hassanain, HH, Sharma, YK, Moldovan, L, Khramtsov, V, Berliner, LJ, Duvick, JP & Goldschmidt-Clermont, P 2000, 'Plant Rac proteins induce superoxide production in mammalian cells', Biochemical and Biophysical Research Communications, vol. 272, no. 3, pp. 783-788. https://doi.org/10.1006/bbrc.2000.2791
Hassanain HH, Sharma YK, Moldovan L, Khramtsov V, Berliner LJ, Duvick JP et al. Plant Rac proteins induce superoxide production in mammalian cells. Biochemical and Biophysical Research Communications. 2000 Jun 16;272(3):783-788. https://doi.org/10.1006/bbrc.2000.2791
Hassanain, Hamdy H. ; Sharma, Yogesh K. ; Moldovan, Leni ; Khramtsov, Valery ; Berliner, Lawrence J. ; Duvick, Jon P. ; Goldschmidt-Clermont, Pascal. / Plant Rac proteins induce superoxide production in mammalian cells. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 272, No. 3. pp. 783-788.
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