TY - JOUR
T1 - Planned variation in preanalytical conditions to evaluate biospecimen stability in the National Children's Study (NCS)
AU - Mechanic, Leah
AU - Mendez, Armando
AU - Merrill, Lori
AU - Rogers, John
AU - Layton, Marnie
AU - Todd, Deborah
AU - Varanasi, Arti
AU - O'Brien, Barbara
AU - Meyer, William A.
AU - Zhang, Ming
AU - Schleicher, Rosemary L.
AU - Moye, Jack
N1 - Funding Information:
stated that there are no conflicts of interest regarding the publication of this article. Research funding played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication. Research funding: This analysis was conducted as part of the National Children’s Study, supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development, and funded, through its appropriation, by the Office of the Director of the National Institutes of Health. Supported by NICHD Contracts HHSN275200503395C, HHSN275201000107U, HHSN275200900010C, and HHSN275201000121U. The manuscript was developed by a Writing Team identified by the National Children’s Study Publications Committee for the purpose of timely sharing of NCS data. Employment or leadership: None declared. Honorarium: None declared. Authors’ declaration: The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention, National Children’s Study, National Institutes of Health, or U.S. Department of Health and Human Services.
PY - 2013/12/1
Y1 - 2013/12/1
N2 - Background: Preanalytical conditions encountered during collection, processing, and storage of biospecimens may influence laboratory results. The National Children's Study (NCS) is a planned prospective cohort study of 100,000 families to examine the influence of a wide variety of exposures on child health. In developing biospecimen collection, processing, and storage procedures for the NCS, we identified several analytes of different biochemical categories for which it was unclear to what extent deviations from NCS procedures could influence measurement results. Methods: A pilot study was performed to examine effects of preanalytic sample handling conditions (delays in centrifugation, freezing delays, delays in separation from cells, additive delay, and tube type) on concentrations of eight different analytes. A total of 2825 measurements were made to assess 15 unique combinations of analyte and handling conditions in blood collected from 151 women of childbearing age (≥20 individuals per handling condition). Results: The majority of analytes were stable under the conditions evaluated. However, levels of plasma interleukin-6 and serum insulin were decreased in response to sample centrifugation delays of up to 5.5 h post-collection (p<0.0001). In addition, delays in freezing centrifuged plasma samples (comparing 24, 48 and 72 h to immediate freezing) resulted in increased levels of adrenocorticotropic hormone (p=0.0014). Conclusions: Determining stability of proposed analytes in response to preanalytical conditions and handling helps to ensure high-quality specimens for study now and in the future. The results inform development of procedures, plans for measurement of analytes, and interpretation of laboratory results.
AB - Background: Preanalytical conditions encountered during collection, processing, and storage of biospecimens may influence laboratory results. The National Children's Study (NCS) is a planned prospective cohort study of 100,000 families to examine the influence of a wide variety of exposures on child health. In developing biospecimen collection, processing, and storage procedures for the NCS, we identified several analytes of different biochemical categories for which it was unclear to what extent deviations from NCS procedures could influence measurement results. Methods: A pilot study was performed to examine effects of preanalytic sample handling conditions (delays in centrifugation, freezing delays, delays in separation from cells, additive delay, and tube type) on concentrations of eight different analytes. A total of 2825 measurements were made to assess 15 unique combinations of analyte and handling conditions in blood collected from 151 women of childbearing age (≥20 individuals per handling condition). Results: The majority of analytes were stable under the conditions evaluated. However, levels of plasma interleukin-6 and serum insulin were decreased in response to sample centrifugation delays of up to 5.5 h post-collection (p<0.0001). In addition, delays in freezing centrifuged plasma samples (comparing 24, 48 and 72 h to immediate freezing) resulted in increased levels of adrenocorticotropic hormone (p=0.0014). Conclusions: Determining stability of proposed analytes in response to preanalytical conditions and handling helps to ensure high-quality specimens for study now and in the future. The results inform development of procedures, plans for measurement of analytes, and interpretation of laboratory results.
KW - Adrenocorticotropic hormone (ACTH)
KW - Biospecimen stability
KW - Carbohydrate-deficient transferrin (CDT)
KW - Estradiol
KW - Free T
KW - IGF-1
KW - IL-6
KW - Insulin
KW - Preanalytical variation
KW - Vitamin C
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U2 - 10.1515/cclm-2013-0454
DO - 10.1515/cclm-2013-0454
M3 - Article
C2 - 23924524
AN - SCOPUS:84889562253
VL - 51
SP - 2287
EP - 2294
JO - Zeitschrift fur klinische Chemie und klinische Biochemie
JF - Zeitschrift fur klinische Chemie und klinische Biochemie
SN - 1434-6621
IS - 12
ER -