Placental transport of brevetoxin-3 in CD-1 mice

Janet M. Benson, Andrea P. Gomez, Gloria L. Statom, Brad M. Tibbetts, Lora E. Fleming, Lorraine C. Backer, Andrew Reich, Daniel G. Baden

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The purpose of this study was to examine the distribution of brevetoxin-3 administered to pregnant dams and to determine the extent of placental transport to fetuses. Twenty-nine pregnant CD-1 mice were administered 3H-brevetoxin-3 (∼1.3 μCi/animal; ∼2.8 μg compound/kg) by intratracheal instillation on one of gestational days 15-18. Groups of four or five dams were killed at selected times through 48 h post-dosing. Four pregnant dams were administered 3H-brevetoxin-3 on gestational day 15 or 16 via osmotic minipump to provide continuous delivery of compound (∼0.13 μCi, 7.5 ng compound/day) over a 72-h period. Then the dams and fetuses were killed. Brevetoxin-associated radioactivity was detected in placentas and fetuses within 0.5 h of intratracheal administration. Concentrations of brevetoxin equivalents in fetuses were approximately 0.3 ng/g throughout the 48-h post-dosing, resulting in a calculated dose to fetuses of 19 ng/g h. Following brevetoxin infusion, concentration of brevetoxin equivalents in fetuses was 0.1 ng/g, lower than that present in most maternal tissues. Results demonstrated placental transport of brevetoxin or its metabolites following maternal acute exposure and repeated low-dose exposure. The consequences of these findings for pregnant women exposed to brevetoxins by inhalation or ingestion remain to be determined.

Original languageEnglish
Pages (from-to)1018-1026
Number of pages9
JournalToxicon
Volume48
Issue number8
DOIs
StatePublished - Dec 15 2006
Externally publishedYes

Fingerprint

Fetus
Dams
Maternal Exposure
Radioactivity
Metabolites
Placenta
Inhalation
brevetoxin 3
brevetoxin
Pregnant Women
Animals
Eating
Mothers
Tissue

Keywords

  • Brevetoxin-3
  • Intratracheal instillation
  • Mice
  • Placental transport

ASJC Scopus subject areas

  • Toxicology

Cite this

Benson, J. M., Gomez, A. P., Statom, G. L., Tibbetts, B. M., Fleming, L. E., Backer, L. C., ... Baden, D. G. (2006). Placental transport of brevetoxin-3 in CD-1 mice. Toxicon, 48(8), 1018-1026. https://doi.org/10.1016/j.toxicon.2006.08.008

Placental transport of brevetoxin-3 in CD-1 mice. / Benson, Janet M.; Gomez, Andrea P.; Statom, Gloria L.; Tibbetts, Brad M.; Fleming, Lora E.; Backer, Lorraine C.; Reich, Andrew; Baden, Daniel G.

In: Toxicon, Vol. 48, No. 8, 15.12.2006, p. 1018-1026.

Research output: Contribution to journalArticle

Benson, JM, Gomez, AP, Statom, GL, Tibbetts, BM, Fleming, LE, Backer, LC, Reich, A & Baden, DG 2006, 'Placental transport of brevetoxin-3 in CD-1 mice', Toxicon, vol. 48, no. 8, pp. 1018-1026. https://doi.org/10.1016/j.toxicon.2006.08.008
Benson JM, Gomez AP, Statom GL, Tibbetts BM, Fleming LE, Backer LC et al. Placental transport of brevetoxin-3 in CD-1 mice. Toxicon. 2006 Dec 15;48(8):1018-1026. https://doi.org/10.1016/j.toxicon.2006.08.008
Benson, Janet M. ; Gomez, Andrea P. ; Statom, Gloria L. ; Tibbetts, Brad M. ; Fleming, Lora E. ; Backer, Lorraine C. ; Reich, Andrew ; Baden, Daniel G. / Placental transport of brevetoxin-3 in CD-1 mice. In: Toxicon. 2006 ; Vol. 48, No. 8. pp. 1018-1026.
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