Abstract
Objective: To measure and compare placental mRNA expression in the maternal circulation among women with intrauterine and ectopic pregnancies. Methods: Plasma was collected from patients in early pregnancy at risk of ectopic pregnancy. Clinical information was prospectively collected and entered into a dedicated database. mRNA was isolated from maternal plasma and quantitative RT-PCR was performed to measure mRNA for human gonadotropin (hCG) and human placental lactogen (hPL). GAPDH mRNA expression was used as an internal control. Results: Twelve women with ectopic pregnancy and 13 women with intrauterine pregnancy were enrolled. Patients with ectopic pregnancy were 6 times more likely to have undetectable levels of hPL mRNA (relative risk [RR] 6.36; 95% confidence interval [CI], 1.70-23.20; P < 0.01). They were also 8 times more likely to have undetectable levels of hCG mRNA (RR 8.64, 95% CI, 1.30-57.10; P < 0.01). mRNA copy numbers for hPL and hCG (normalized by GAPDH) were significantly lower in the ectopic group than in the intrauterine group. Conclusion: Placental mRNA is present in the maternal circulation in significantly lower copies in cases of ectopic pregnancy compared with cases of intrauterine pregnancy. Measurement of placental mRNA in the maternal circulation may help to distinguish between intrauterine and ectopic pregnancies.
| Original language | English |
|---|---|
| Pages (from-to) | 131-133 |
| Number of pages | 3 |
| Journal | International Journal of Gynecology and Obstetrics |
| Volume | 117 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 1 2012 |
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Keywords
- Ectopic pregnancy
- Intrauterine pregnancy
- Placental mRNA
ASJC Scopus subject areas
- Obstetrics and Gynecology
Cite this
Placental mRNA in maternal plasma as a predictor of ectopic pregnancy. / Takacs, Peter; Jaramillo, Sindy; Datar, Ram; Williams, Anthony; Olczyk, Joseph; Barnhart, Kurt.
In: International Journal of Gynecology and Obstetrics, Vol. 117, No. 2, 01.05.2012, p. 131-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Placental mRNA in maternal plasma as a predictor of ectopic pregnancy
AU - Takacs, Peter
AU - Jaramillo, Sindy
AU - Datar, Ram
AU - Williams, Anthony
AU - Olczyk, Joseph
AU - Barnhart, Kurt
PY - 2012/5/1
Y1 - 2012/5/1
N2 - Objective: To measure and compare placental mRNA expression in the maternal circulation among women with intrauterine and ectopic pregnancies. Methods: Plasma was collected from patients in early pregnancy at risk of ectopic pregnancy. Clinical information was prospectively collected and entered into a dedicated database. mRNA was isolated from maternal plasma and quantitative RT-PCR was performed to measure mRNA for human gonadotropin (hCG) and human placental lactogen (hPL). GAPDH mRNA expression was used as an internal control. Results: Twelve women with ectopic pregnancy and 13 women with intrauterine pregnancy were enrolled. Patients with ectopic pregnancy were 6 times more likely to have undetectable levels of hPL mRNA (relative risk [RR] 6.36; 95% confidence interval [CI], 1.70-23.20; P < 0.01). They were also 8 times more likely to have undetectable levels of hCG mRNA (RR 8.64, 95% CI, 1.30-57.10; P < 0.01). mRNA copy numbers for hPL and hCG (normalized by GAPDH) were significantly lower in the ectopic group than in the intrauterine group. Conclusion: Placental mRNA is present in the maternal circulation in significantly lower copies in cases of ectopic pregnancy compared with cases of intrauterine pregnancy. Measurement of placental mRNA in the maternal circulation may help to distinguish between intrauterine and ectopic pregnancies.
AB - Objective: To measure and compare placental mRNA expression in the maternal circulation among women with intrauterine and ectopic pregnancies. Methods: Plasma was collected from patients in early pregnancy at risk of ectopic pregnancy. Clinical information was prospectively collected and entered into a dedicated database. mRNA was isolated from maternal plasma and quantitative RT-PCR was performed to measure mRNA for human gonadotropin (hCG) and human placental lactogen (hPL). GAPDH mRNA expression was used as an internal control. Results: Twelve women with ectopic pregnancy and 13 women with intrauterine pregnancy were enrolled. Patients with ectopic pregnancy were 6 times more likely to have undetectable levels of hPL mRNA (relative risk [RR] 6.36; 95% confidence interval [CI], 1.70-23.20; P < 0.01). They were also 8 times more likely to have undetectable levels of hCG mRNA (RR 8.64, 95% CI, 1.30-57.10; P < 0.01). mRNA copy numbers for hPL and hCG (normalized by GAPDH) were significantly lower in the ectopic group than in the intrauterine group. Conclusion: Placental mRNA is present in the maternal circulation in significantly lower copies in cases of ectopic pregnancy compared with cases of intrauterine pregnancy. Measurement of placental mRNA in the maternal circulation may help to distinguish between intrauterine and ectopic pregnancies.
KW - Ectopic pregnancy
KW - Intrauterine pregnancy
KW - Placental mRNA
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UR - http://www.scopus.com/inward/citedby.url?scp=84859597986&partnerID=8YFLogxK
U2 - 10.1016/j.ijgo.2011.12.011
DO - 10.1016/j.ijgo.2011.12.011
M3 - Article
VL - 117
SP - 131
EP - 133
JO - International Journal of Gynecology and Obstetrics
JF - International Journal of Gynecology and Obstetrics
SN - 0020-7292
IS - 2
ER -