Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats

Lars C. Moeller, Manuela Alonso, XiaoHui Liao, Vance Broach, Alexandra Dumitrescu, Jacqueline Van Sande, Lucia Montanelli, Stephen Skjei, Charles Goodwin, Helmut Grasberger, Samuel Refetoff, Roy E Weiss

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The genetic basis for differences in TSH sensitivity between two rat strains was examined using consomic rats generated from original strains salt-sensitive Dahl (SS) (TSH 1.8 ± 0.1 ng/ml; free T4 index 4.9 ± 0.4) and Brown Norwegian (BN) (TSH 5.5 ± 0.6 ng/ml, P < 0.05; free T4 index 4.3 ± 0.1, P not significant). Consomic rats SSBN6 [BN chromosome (CH) 6 placed in SS rat] and SSBN2 (BN CH 2 placed in SS rat) have TSH concentrations intermediate between pure SS and BN strains (2.9 ± 0.3 and 3.1 ± 0.3 ng/ml, respectively; P< 0.05). Candidate genes on rat CH2 included TSH β-subunit and on CH6 the TSH receptor (TSHR). TSH from sera of BN, SS, SSBN6, and SSBN2 strains had similar in vitro bioactivity suggesting that the cause for the variable TSH concentrations was not due to an altered TSH. Physiological response to TSH was measured by changes in serum T4 concentrations upon administration of bovine TSH (bTSH). Rat strain SS had a greater T4 response to bTSH than BN (change in T4, 1.3 ± 0.1 vs. 0.4 ± 0.1 μg/dl, P < 0.005), suggesting reduced thyrocyte sensitivity to TSH in BN. Sequencing of the TSHR coding region revealed an amino acid difference in BN (Q46R). This substitution is unlikely to contribute to the strain difference in serum TSH because both TSHR variants were equally expressed at the cell surface of transfected cells and responsive to bTSH. Given similar TSH activity and similar TSHR structure, TSHR mRNA expression in thyroid tissue was quantitated by real-time PCR. BN had 54 ± 5% the total TSHR expression compared to SS (100 ± 7%, P < 0.0001), when corrected for GAPDH expression, a difference confirmed at the protein level. Therefore, the higher TSH level in the BN strain appears to reflect an adjustment of the feedback loop to reduced thyrocyte sensitivity to TSH secondary to reduced TSHR expression. These strains of rat provide a model to study the cis- and trans-acting factors underlying the difference in TSHR expression.

Original languageEnglish (US)
Pages (from-to)4727-4733
Number of pages7
JournalEndocrinology
Volume148
Issue number10
DOIs
StatePublished - Oct 2007
Externally publishedYes

Fingerprint

Thyrotropin Receptors
Thyroid Gland
Inbred Dahl Rats
Salts
Serum
Chromosomes, Human, Pair 6
Trans-Activators
Chromosomes, Human, Pair 2
Real-Time Polymerase Chain Reaction
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Moeller, L. C., Alonso, M., Liao, X., Broach, V., Dumitrescu, A., Van Sande, J., ... Weiss, R. E. (2007). Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats. Endocrinology, 148(10), 4727-4733. https://doi.org/10.1210/en.2007-0236

Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats. / Moeller, Lars C.; Alonso, Manuela; Liao, XiaoHui; Broach, Vance; Dumitrescu, Alexandra; Van Sande, Jacqueline; Montanelli, Lucia; Skjei, Stephen; Goodwin, Charles; Grasberger, Helmut; Refetoff, Samuel; Weiss, Roy E.

In: Endocrinology, Vol. 148, No. 10, 10.2007, p. 4727-4733.

Research output: Contribution to journalArticle

Moeller, LC, Alonso, M, Liao, X, Broach, V, Dumitrescu, A, Van Sande, J, Montanelli, L, Skjei, S, Goodwin, C, Grasberger, H, Refetoff, S & Weiss, RE 2007, 'Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats', Endocrinology, vol. 148, no. 10, pp. 4727-4733. https://doi.org/10.1210/en.2007-0236
Moeller LC, Alonso M, Liao X, Broach V, Dumitrescu A, Van Sande J et al. Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats. Endocrinology. 2007 Oct;148(10):4727-4733. https://doi.org/10.1210/en.2007-0236
Moeller, Lars C. ; Alonso, Manuela ; Liao, XiaoHui ; Broach, Vance ; Dumitrescu, Alexandra ; Van Sande, Jacqueline ; Montanelli, Lucia ; Skjei, Stephen ; Goodwin, Charles ; Grasberger, Helmut ; Refetoff, Samuel ; Weiss, Roy E. / Pituitary-thyroid setpoint and thyrotropin receptor expression in consomic rats. In: Endocrinology. 2007 ; Vol. 148, No. 10. pp. 4727-4733.
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AU - Van Sande, Jacqueline

AU - Montanelli, Lucia

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