Piccolo, a presynaptic zinc finger protein structurally related to Bassoon

Steven D. Fenster, Wook Joon Chung, Rong G Zhai, Claudia Cases-Langhoff, Britta Voss, Abigail M. Garner, Udo Kaempf, Stefan Kindler, Eckart D. Gundelfinger, Craig C. Garner

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Analysis of its primary structure reveals that Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins were found to be shared components of glutamatergic and GABAergic CNS synapses but not of the cholinergic neuromuscular junction. The Piccolo zinc fingers were found to interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. We show that PRA1 is a synaptic vesicle-associated protein that is colocalized with Piccolo in nerve terminals of hippocampal primary neurons. These data suggest that Piccolo plays a role in the trafficking of synaptic vesicles (SVs) at the active zone.

Original languageEnglish
Pages (from-to)203-214
Number of pages12
JournalNeuron
Volume25
Issue number1
StatePublished - Jan 1 2000
Externally publishedYes

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Zinc Fingers
Synaptic Vesicles
Vesicle-Associated Membrane Protein 2
R-SNARE Proteins
Proteins
Cytoskeletal Proteins
Neuromuscular Junction
Synapses
Cholinergic Agents
Neurotransmitter Agents
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fenster, S. D., Chung, W. J., Zhai, R. G., Cases-Langhoff, C., Voss, B., Garner, A. M., ... Garner, C. C. (2000). Piccolo, a presynaptic zinc finger protein structurally related to Bassoon. Neuron, 25(1), 203-214.

Piccolo, a presynaptic zinc finger protein structurally related to Bassoon. / Fenster, Steven D.; Chung, Wook Joon; Zhai, Rong G; Cases-Langhoff, Claudia; Voss, Britta; Garner, Abigail M.; Kaempf, Udo; Kindler, Stefan; Gundelfinger, Eckart D.; Garner, Craig C.

In: Neuron, Vol. 25, No. 1, 01.01.2000, p. 203-214.

Research output: Contribution to journalArticle

Fenster, SD, Chung, WJ, Zhai, RG, Cases-Langhoff, C, Voss, B, Garner, AM, Kaempf, U, Kindler, S, Gundelfinger, ED & Garner, CC 2000, 'Piccolo, a presynaptic zinc finger protein structurally related to Bassoon', Neuron, vol. 25, no. 1, pp. 203-214.
Fenster SD, Chung WJ, Zhai RG, Cases-Langhoff C, Voss B, Garner AM et al. Piccolo, a presynaptic zinc finger protein structurally related to Bassoon. Neuron. 2000 Jan 1;25(1):203-214.
Fenster, Steven D. ; Chung, Wook Joon ; Zhai, Rong G ; Cases-Langhoff, Claudia ; Voss, Britta ; Garner, Abigail M. ; Kaempf, Udo ; Kindler, Stefan ; Gundelfinger, Eckart D. ; Garner, Craig C. / Piccolo, a presynaptic zinc finger protein structurally related to Bassoon. In: Neuron. 2000 ; Vol. 25, No. 1. pp. 203-214.
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