Piccolo, a presynaptic zinc finger protein structurally related to Bassoon

Steven D. Fenster, Wook Joon Chung, Rong Zhai, Claudia Cases-Langhoff, Britta Voss, Abigail M. Garner, Udo Kaempf, Stefan Kindler, Eckart D. Gundelfinger, Craig C. Garner

Research output: Contribution to journalArticle

208 Scopus citations

Abstract

Piccolo is a novel component of the presynaptic cytoskeletal matrix (PCM) assembled at the active zone of neurotransmitter release. Analysis of its primary structure reveals that Piccolo is a multidomain zinc finger protein structurally related to Bassoon, another PCM protein. Both proteins were found to be shared components of glutamatergic and GABAergic CNS synapses but not of the cholinergic neuromuscular junction. The Piccolo zinc fingers were found to interact with the dual prenylated rab3A and VAMP2/Synaptobrevin II receptor PRA1. We show that PRA1 is a synaptic vesicle-associated protein that is colocalized with Piccolo in nerve terminals of hippocampal primary neurons. These data suggest that Piccolo plays a role in the trafficking of synaptic vesicles (SVs) at the active zone.

Original languageEnglish (US)
Pages (from-to)203-214
Number of pages12
JournalNeuron
Volume25
Issue number1
DOIs
StatePublished - Jan 2000
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fenster, S. D., Chung, W. J., Zhai, R., Cases-Langhoff, C., Voss, B., Garner, A. M., Kaempf, U., Kindler, S., Gundelfinger, E. D., & Garner, C. C. (2000). Piccolo, a presynaptic zinc finger protein structurally related to Bassoon. Neuron, 25(1), 203-214. https://doi.org/10.1016/S0896-6273(00)80883-1