Physiological responses to acute silver exposure in the freshwater crayfish (cambarus diogenes diogenes) - A model invertebrate?

Martin Grosell, Colin J. Brauner, Scott P. Kelly, James C. McGeer, Adalto Bianchini, Chris M. Wood

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Adult crayfish (Cambarus diogenes diogenes) exposed to 8.41 ± 0.17 μg silver/L (19.4% as Ag+) in moderately hard freshwater under flow-through conditions for 96 h exhibited ionoregulatory disturbance, elevated metabolic ammonia (Tamm) production and substantial silver accumulation in the gills, hemolymph, and hepatopancreas. The ionoregulatory disturbance included both a generally reduced unidirectional Na+ influx and an increased unidirectional Na+ efflux, leading to a substantial net loss of Na+ from the silver-exposed crayfish. The Na+ uptake in silver-exposed crayfish differed overall from controls, while the increased Na+ efflux recovered to control values 48 h into the 96 h of exposure. The general inhibition of Na+ uptake could be explained by a reduced sodium/potassium-adenosine triphosphatase (Na/K-ATPase) activity in terminally obtained gill samples from the silver-exposed crayfish. The silver-induced effect on Na+ uptake and loss translated to reduced hemolymph Na+ concentrations but not significantly reduced hemolymph Cl- concentrations. Hemolymph Tamm and Tamm efflux both increased in silver-exposed crayfish, indicating an increased metabolic Tamm production. The present study demonstrates that the toxic mechanism of waterborne silver exposure in freshwater crayfish resembles that of freshwater teleost fish. The crayfish might therefore be a useful model system for extending current environmental regulatory strategies, currently based on teleost fish, to invertebrates.

Original languageEnglish
Pages (from-to)369-374
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume21
Issue number2
StatePublished - Jan 29 2002
Externally publishedYes

Fingerprint

Astacoidea
Invertebrates
physiological response
crayfish
Fresh Water
Silver
silver
invertebrate
Hemolymph
teleost
Fish
Fishes
Hepatopancreas
disturbance
Sodium-Potassium-Exchanging ATPase
exposure
Poisons
fish
Ammonia
Adenosine Triphosphatases

Keywords

  • Crayfish
  • Osmoregulatory disturbance
  • Silver accumulation
  • Silver toxicity
  • Unidirectional Na flux

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Physiological responses to acute silver exposure in the freshwater crayfish (cambarus diogenes diogenes) - A model invertebrate? / Grosell, Martin; Brauner, Colin J.; Kelly, Scott P.; McGeer, James C.; Bianchini, Adalto; Wood, Chris M.

In: Environmental Toxicology and Chemistry, Vol. 21, No. 2, 29.01.2002, p. 369-374.

Research output: Contribution to journalArticle

Grosell, Martin ; Brauner, Colin J. ; Kelly, Scott P. ; McGeer, James C. ; Bianchini, Adalto ; Wood, Chris M. / Physiological responses to acute silver exposure in the freshwater crayfish (cambarus diogenes diogenes) - A model invertebrate?. In: Environmental Toxicology and Chemistry. 2002 ; Vol. 21, No. 2. pp. 369-374.
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