Physiological and biochemical properties of a temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) and revertant from Chinese hamster cells

Rosann A. Farber, Murray P. Deutscher

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

A relevant (rl) of the temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) of Chinese hamster ovary cells has been analyzed. The enzyme in the revertant is also temperature sensitive, but its activity at every temperature tested is slightly higher than that of the mutant. Both tsHl and the revertant are sensitive to a decreased concentration of leucine in the growth medium, but the revertant is less sensitive than the mutant. The tsHl synthetase has a Km for leucine which is 80-fold higher at 39°C than that of wildtype CHO cells, whereas the Km of the rl enzyme is 20-fold higher than the wild type at this temperature.

Original languageEnglish (US)
Pages (from-to)509-520
Number of pages12
JournalSomatic Cell Genetics
Volume2
Issue number6
DOIs
StatePublished - Nov 1 1976

ASJC Scopus subject areas

  • Genetics

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