Physiological and biochemical properties of a temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) and revertant from Chinese hamster cells

Rosann A. Farber; Murray P. Deutscher

doi:10.1007/BF01542688

Physiological and biochemical properties of a temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) and revertant from Chinese hamster cells

Rosann A. Farber, Murray P. Deutscher

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A relevant (rl) of the temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) of Chinese hamster ovary cells has been analyzed. The enzyme in the revertant is also temperature sensitive, but its activity at every temperature tested is slightly higher than that of the mutant. Both tsHl and the revertant are sensitive to a decreased concentration of leucine in the growth medium, but the revertant is less sensitive than the mutant. The tsHl synthetase has a K_m for leucine which is 80-fold higher at 39°C than that of wildtype CHO cells, whereas the K_m of the rl enzyme is 20-fold higher than the wild type at this temperature.

Original language	English (US)
Pages (from-to)	509-520
Number of pages	12
Journal	Somatic Cell Genetics
Volume	2
Issue number	6
DOIs	https://doi.org/10.1007/BF01542688
State	Published - Nov 1 1976

ASJC Scopus subject areas

Genetics

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title = "Physiological and biochemical properties of a temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) and revertant from Chinese hamster cells",

abstract = "A relevant (rl) of the temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) of Chinese hamster ovary cells has been analyzed. The enzyme in the revertant is also temperature sensitive, but its activity at every temperature tested is slightly higher than that of the mutant. Both tsHl and the revertant are sensitive to a decreased concentration of leucine in the growth medium, but the revertant is less sensitive than the mutant. The tsHl synthetase has a Km for leucine which is 80-fold higher at 39°C than that of wildtype CHO cells, whereas the Km of the rl enzyme is 20-fold higher than the wild type at this temperature.",

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AU - Farber, Rosann A.

AU - Deutscher, Murray P.

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N2 - A relevant (rl) of the temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) of Chinese hamster ovary cells has been analyzed. The enzyme in the revertant is also temperature sensitive, but its activity at every temperature tested is slightly higher than that of the mutant. Both tsHl and the revertant are sensitive to a decreased concentration of leucine in the growth medium, but the revertant is less sensitive than the mutant. The tsHl synthetase has a Km for leucine which is 80-fold higher at 39°C than that of wildtype CHO cells, whereas the Km of the rl enzyme is 20-fold higher than the wild type at this temperature.

AB - A relevant (rl) of the temperature-sensitive leucyl-tRNA synthetase mutant (tsHl) of Chinese hamster ovary cells has been analyzed. The enzyme in the revertant is also temperature sensitive, but its activity at every temperature tested is slightly higher than that of the mutant. Both tsHl and the revertant are sensitive to a decreased concentration of leucine in the growth medium, but the revertant is less sensitive than the mutant. The tsHl synthetase has a Km for leucine which is 80-fold higher at 39°C than that of wildtype CHO cells, whereas the Km of the rl enzyme is 20-fold higher than the wild type at this temperature.

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