Physical characterization of high-affinity gastrointestinal Cu transport in vitro in freshwater rainbow trout Oncorhynchus mykiss

Sunita R. Nadella, Martin Grosell, Chris M. Wood

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

This study investigated the transport of copper (Cu) in the gut of trout. Examination of the spatial distribution of Cu along the digestive tract and a physical characterization of the uptake process was carried out using an in vitro gut sac technique and 64Cu as a tracer. Unidirectional Cu uptake was highest in the anterior intestine followed in decreasing order by the posterior intestine, mid intestine and the stomach. Cu uptake was resistant to hypoxia and appeared to be fueled equally well by Cu(II) or Cu (I) at Cu concentrations typically found in the fluid phase of the chyme in vivo in the trout intestine. Transport demonstrated saturation kinetics (e.g. K m = 31.6 μM, J max = 17 pmol cm-2 h-1, in mid intestine) at low Cu levels representative of those measured in the chyme in vivo, with a diffusive component at higher Cu concentrations. Q 10 analysis indicated Cu uptake is via diffusion across the apical membrane and biologically mediated across the basolateral membranes of enterocytes. The presence of l-histidine but not d-histidine stimulated both Cu and Na uptake suggesting a common pathway for the transport of Cu/Na with L-histidine.

Original languageEnglish
Pages (from-to)793-806
Number of pages14
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume176
Issue number8
DOIs
StatePublished - Nov 1 2006

Fingerprint

Oncorhynchus mykiss
Fresh Water
Histidine
rainbow
Intestines
intestines
membrane
histidine
hypoxia
Trout
Membranes
tracer
trout
saturation
spatial distribution
copper
kinetics
digestive system
Spatial distribution
fluid

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • Physiology
  • Physiology (medical)

Cite this

@article{5e101441f84449f5a57c2ff6e94f4249,
title = "Physical characterization of high-affinity gastrointestinal Cu transport in vitro in freshwater rainbow trout Oncorhynchus mykiss",
abstract = "This study investigated the transport of copper (Cu) in the gut of trout. Examination of the spatial distribution of Cu along the digestive tract and a physical characterization of the uptake process was carried out using an in vitro gut sac technique and 64Cu as a tracer. Unidirectional Cu uptake was highest in the anterior intestine followed in decreasing order by the posterior intestine, mid intestine and the stomach. Cu uptake was resistant to hypoxia and appeared to be fueled equally well by Cu(II) or Cu (I) at Cu concentrations typically found in the fluid phase of the chyme in vivo in the trout intestine. Transport demonstrated saturation kinetics (e.g. K m = 31.6 μM, J max = 17 pmol cm-2 h-1, in mid intestine) at low Cu levels representative of those measured in the chyme in vivo, with a diffusive component at higher Cu concentrations. Q 10 analysis indicated Cu uptake is via diffusion across the apical membrane and biologically mediated across the basolateral membranes of enterocytes. The presence of l-histidine but not d-histidine stimulated both Cu and Na uptake suggesting a common pathway for the transport of Cu/Na with L-histidine.",
author = "Nadella, {Sunita R.} and Martin Grosell and Wood, {Chris M.}",
year = "2006",
month = "11",
day = "1",
doi = "10.1007/s00360-006-0101-z",
language = "English",
volume = "176",
pages = "793--806",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
issn = "0174-1578",
publisher = "Springer Verlag",
number = "8",

}

TY - JOUR

T1 - Physical characterization of high-affinity gastrointestinal Cu transport in vitro in freshwater rainbow trout Oncorhynchus mykiss

AU - Nadella, Sunita R.

AU - Grosell, Martin

AU - Wood, Chris M.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - This study investigated the transport of copper (Cu) in the gut of trout. Examination of the spatial distribution of Cu along the digestive tract and a physical characterization of the uptake process was carried out using an in vitro gut sac technique and 64Cu as a tracer. Unidirectional Cu uptake was highest in the anterior intestine followed in decreasing order by the posterior intestine, mid intestine and the stomach. Cu uptake was resistant to hypoxia and appeared to be fueled equally well by Cu(II) or Cu (I) at Cu concentrations typically found in the fluid phase of the chyme in vivo in the trout intestine. Transport demonstrated saturation kinetics (e.g. K m = 31.6 μM, J max = 17 pmol cm-2 h-1, in mid intestine) at low Cu levels representative of those measured in the chyme in vivo, with a diffusive component at higher Cu concentrations. Q 10 analysis indicated Cu uptake is via diffusion across the apical membrane and biologically mediated across the basolateral membranes of enterocytes. The presence of l-histidine but not d-histidine stimulated both Cu and Na uptake suggesting a common pathway for the transport of Cu/Na with L-histidine.

AB - This study investigated the transport of copper (Cu) in the gut of trout. Examination of the spatial distribution of Cu along the digestive tract and a physical characterization of the uptake process was carried out using an in vitro gut sac technique and 64Cu as a tracer. Unidirectional Cu uptake was highest in the anterior intestine followed in decreasing order by the posterior intestine, mid intestine and the stomach. Cu uptake was resistant to hypoxia and appeared to be fueled equally well by Cu(II) or Cu (I) at Cu concentrations typically found in the fluid phase of the chyme in vivo in the trout intestine. Transport demonstrated saturation kinetics (e.g. K m = 31.6 μM, J max = 17 pmol cm-2 h-1, in mid intestine) at low Cu levels representative of those measured in the chyme in vivo, with a diffusive component at higher Cu concentrations. Q 10 analysis indicated Cu uptake is via diffusion across the apical membrane and biologically mediated across the basolateral membranes of enterocytes. The presence of l-histidine but not d-histidine stimulated both Cu and Na uptake suggesting a common pathway for the transport of Cu/Na with L-histidine.

UR - http://www.scopus.com/inward/record.url?scp=33750050799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750050799&partnerID=8YFLogxK

U2 - 10.1007/s00360-006-0101-z

DO - 10.1007/s00360-006-0101-z

M3 - Article

VL - 176

SP - 793

EP - 806

JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

SN - 0174-1578

IS - 8

ER -