Physical and cDNA mapping in the DBH region of human chromosome 9q34

John Gilbert, A. Kumar, S. Newey, N. Rao, P. Ioannou, H. Qiu, D. Lin, P. Xu, M. J. Pettenati, Margaret A Pericak-Vance

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Chromosome 9q34 has been extensively studied and mapped due to the presence of known disease genes, principally tuberous sclerosis 1 (TSC1), in this region. During the course of our mapping of this region we constructed a 555-kb contig beginning approximately 50 kb proximal to the dopamine-β-hydroxylase (DBH) gene and extending, with one small deletion, distal to the D9S 114 marker. The contig consists of 11 P1 clones, four PAC clones, one BAC clone and six cosmid clones and contains 27 new nonpolymorphic STSs. We have found the region to be unstable in P1, PAC and BAC cloning vehicles and have identified several deleted genomic clones. In addition, we have isolated and mapped the 3' portions of three putative genes located within or immediately distal to the DBH gene, including one large gene that runs on the opposite strand to DBH and utilizes portions of two DBH exons. The genomic clones of the contig, cDNAs and new STSs will be useful reagents for the further study and mapping of this region. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalHuman Heredity
Volume50
Issue number3
StatePublished - May 1 2000
Externally publishedYes

Fingerprint

Human Chromosomes
Complementary DNA
Clone Cells
Genes
Cosmids
Mixed Function Oxygenases
Organism Cloning
Exons
Dopamine
Chromosomes

Keywords

  • cDNA
  • Genomic contig
  • Human chromosome 9q

ASJC Scopus subject areas

  • Genetics(clinical)

Cite this

Gilbert, J., Kumar, A., Newey, S., Rao, N., Ioannou, P., Qiu, H., ... Pericak-Vance, M. A. (2000). Physical and cDNA mapping in the DBH region of human chromosome 9q34. Human Heredity, 50(3), 151-157.

Physical and cDNA mapping in the DBH region of human chromosome 9q34. / Gilbert, John; Kumar, A.; Newey, S.; Rao, N.; Ioannou, P.; Qiu, H.; Lin, D.; Xu, P.; Pettenati, M. J.; Pericak-Vance, Margaret A.

In: Human Heredity, Vol. 50, No. 3, 01.05.2000, p. 151-157.

Research output: Contribution to journalArticle

Gilbert, J, Kumar, A, Newey, S, Rao, N, Ioannou, P, Qiu, H, Lin, D, Xu, P, Pettenati, MJ & Pericak-Vance, MA 2000, 'Physical and cDNA mapping in the DBH region of human chromosome 9q34', Human Heredity, vol. 50, no. 3, pp. 151-157.
Gilbert J, Kumar A, Newey S, Rao N, Ioannou P, Qiu H et al. Physical and cDNA mapping in the DBH region of human chromosome 9q34. Human Heredity. 2000 May 1;50(3):151-157.
Gilbert, John ; Kumar, A. ; Newey, S. ; Rao, N. ; Ioannou, P. ; Qiu, H. ; Lin, D. ; Xu, P. ; Pettenati, M. J. ; Pericak-Vance, Margaret A. / Physical and cDNA mapping in the DBH region of human chromosome 9q34. In: Human Heredity. 2000 ; Vol. 50, No. 3. pp. 151-157.
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