Photothrombotic occlusion of rat middle cerebral artery: Histopathological and hemodynamic sequelae of acute recanalization

H. Nakayama, W. D. Dietrich, B. D. Watson, R. Busto, M. D. Ginsberg

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


The histopathological and hemodynamic consequences of photochemically induced middle cerebral artery (MCA) thrombosis and recanalization were studied in the rat. Recanalization of the thrombosed MCA segment was achieved by the topical application of nimodipine at 1 h following photochemically induced occlusion. Pathological consequences of permanent and temporary occlusion were compared by morphometric procedures 7 days following thrombus formation. Rats with permanent thrombosis exhibited consistent infarction of both striatum and cortex. MCA recanalization at 1 h was associated with a significant reduction in total infarct volume. In recanalized rats, small cortical infarcts, infarction and ischemic cell damage was documented throughout the striatum in all rats. Local CBF (lCBF), measured autoradiographically, was significantly reduced in the MCA territory following 1 h of MCA occlusion, especially within the striatum. At 1 h after recanalization, lCBF recovered within the previously ischemic brain regions to >50% of control. Perfusion deficits were detected by carbon black infusion within focal areas of the striatum following reperfusion. Thus, cortical neurons appear to tolerate 1 h of MCA occlusion in this model. In contrast, reperfusion following 1 h of photochemically induced MCA occlusion gives rise to selective injury to the striatum.

Original languageEnglish (US)
Pages (from-to)357-366
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number3
StatePublished - 1988

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine


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