Photoacoustic spectra at room and 85 K temperatures as well as photothermal beam deflection spectra of bacteriochlorophyll - lipoprotein complexes from purple bacterium Chromatium minutissimum were measured. Spectra were compared and obtained differences were tentatively explained by various inertion of these two methods. Photothermal beam deflection method measure the heat which is generated in close surroundings of absorbing pigment molecule, whereas usage of more inert photoacoustic signal is averaged over contributions from various pigments located in a larger sample volume and therefore is similar to absorption spectra.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and biophysical research communications|
|State||Published - Jul 15 1992|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology