PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro

Lucie Lorrain, Michel Havaux, Alain Tessier, Roger Leblanc

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Abstract— This Technical Note describes the design of a photothermal beam deflection apparatus which allows the easy and rapid measurement of thermal dissipation of absorbed light energy in various photosynthetic materials including whole plant leaves. This system is based on the “mirage effect” in which the refractive index gradient induced in a fluid in contact with the sample, irradiated with an intensity‐modulated light, causes the periodic deflection of a laser beam parallel to the sample surface. The deflection of the probe laser beam is detected by a position sensor, the output of which is processed by a lock‐in amplifier. Photothermal deflection signals can be monitored in vivo in intact leaves placed in various (liquid or gaseous) environments with a satisfactory signal‐to‐noise ratio between 100 (in water) and 50 (in air) at low modulation frequencies (ca 30 Hz). It is shown that this new and simple photothermal technique is a very sensitive tool for the measurement of absorption spectra of photosynthetic pigments both in vivo (leaves, algae or chloroplasts) and in model systems (Langmuir‐Blodgett and solid films of chlorophyll).

Original languageEnglish (US)
Pages (from-to)491-496
Number of pages6
JournalPhotochemistry and Photobiology
Volume51
Issue number4
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

Fingerprint

photothermal deflection spectroscopy
pigments
Pigments
Laser beams
deflection
Spectrum Analysis
Lasers
Spectroscopy
leaves
Light
Refractometry
Plant Leaves
Frequency modulation
Chloroplasts
Chlorophyll
Algae
Contacts (fluid mechanics)
Absorption spectra
Refractive index
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro. / Lorrain, Lucie; Havaux, Michel; Tessier, Alain; Leblanc, Roger.

In: Photochemistry and Photobiology, Vol. 51, No. 4, 01.01.1990, p. 491-496.

Research output: Contribution to journalArticle

Lorrain, Lucie ; Havaux, Michel ; Tessier, Alain ; Leblanc, Roger. / PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro. In: Photochemistry and Photobiology. 1990 ; Vol. 51, No. 4. pp. 491-496.
@article{d71365f35ced4bf4ab4ba4375e653dd8,
title = "PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro",
abstract = "Abstract— This Technical Note describes the design of a photothermal beam deflection apparatus which allows the easy and rapid measurement of thermal dissipation of absorbed light energy in various photosynthetic materials including whole plant leaves. This system is based on the “mirage effect” in which the refractive index gradient induced in a fluid in contact with the sample, irradiated with an intensity‐modulated light, causes the periodic deflection of a laser beam parallel to the sample surface. The deflection of the probe laser beam is detected by a position sensor, the output of which is processed by a lock‐in amplifier. Photothermal deflection signals can be monitored in vivo in intact leaves placed in various (liquid or gaseous) environments with a satisfactory signal‐to‐noise ratio between 100 (in water) and 50 (in air) at low modulation frequencies (ca 30 Hz). It is shown that this new and simple photothermal technique is a very sensitive tool for the measurement of absorption spectra of photosynthetic pigments both in vivo (leaves, algae or chloroplasts) and in model systems (Langmuir‐Blodgett and solid films of chlorophyll).",
author = "Lucie Lorrain and Michel Havaux and Alain Tessier and Roger Leblanc",
year = "1990",
month = "1",
day = "1",
doi = "10.1111/j.1751-1097.1990.tb01743.x",
language = "English (US)",
volume = "51",
pages = "491--496",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - PHOTOTHERMAL DEFLECTION SPECTROSCOPY OF PHOTOSYNTHETIC PIGMENTS in vivo and in vitro

AU - Lorrain, Lucie

AU - Havaux, Michel

AU - Tessier, Alain

AU - Leblanc, Roger

PY - 1990/1/1

Y1 - 1990/1/1

N2 - Abstract— This Technical Note describes the design of a photothermal beam deflection apparatus which allows the easy and rapid measurement of thermal dissipation of absorbed light energy in various photosynthetic materials including whole plant leaves. This system is based on the “mirage effect” in which the refractive index gradient induced in a fluid in contact with the sample, irradiated with an intensity‐modulated light, causes the periodic deflection of a laser beam parallel to the sample surface. The deflection of the probe laser beam is detected by a position sensor, the output of which is processed by a lock‐in amplifier. Photothermal deflection signals can be monitored in vivo in intact leaves placed in various (liquid or gaseous) environments with a satisfactory signal‐to‐noise ratio between 100 (in water) and 50 (in air) at low modulation frequencies (ca 30 Hz). It is shown that this new and simple photothermal technique is a very sensitive tool for the measurement of absorption spectra of photosynthetic pigments both in vivo (leaves, algae or chloroplasts) and in model systems (Langmuir‐Blodgett and solid films of chlorophyll).

AB - Abstract— This Technical Note describes the design of a photothermal beam deflection apparatus which allows the easy and rapid measurement of thermal dissipation of absorbed light energy in various photosynthetic materials including whole plant leaves. This system is based on the “mirage effect” in which the refractive index gradient induced in a fluid in contact with the sample, irradiated with an intensity‐modulated light, causes the periodic deflection of a laser beam parallel to the sample surface. The deflection of the probe laser beam is detected by a position sensor, the output of which is processed by a lock‐in amplifier. Photothermal deflection signals can be monitored in vivo in intact leaves placed in various (liquid or gaseous) environments with a satisfactory signal‐to‐noise ratio between 100 (in water) and 50 (in air) at low modulation frequencies (ca 30 Hz). It is shown that this new and simple photothermal technique is a very sensitive tool for the measurement of absorption spectra of photosynthetic pigments both in vivo (leaves, algae or chloroplasts) and in model systems (Langmuir‐Blodgett and solid films of chlorophyll).

UR - http://www.scopus.com/inward/record.url?scp=85032069257&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85032069257&partnerID=8YFLogxK

U2 - 10.1111/j.1751-1097.1990.tb01743.x

DO - 10.1111/j.1751-1097.1990.tb01743.x

M3 - Article

VL - 51

SP - 491

EP - 496

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 4

ER -