Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product

Nicholas Tsinoremas, A. M. Castets, M. A. Harrison, J. F. Allen, N. Tandeau De Marsac

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A glnB gene is identified in the cyanobacterium Synechococcus sp. PCC 7942, and its gene product is found to be covalently modified as a result of imbalance in electron transfer in photosynthesis, where photosystem II is favored over photosystem I. The gene was cloned and sequenced and found to encode a polypeptide of 112 amino acid residues, whose sequence shows a high degree of similarity to the Escherichia coli regulatory protein, PII. In E. coli, PII is involved in signal transduction in transcriptional and post-translational regulation of nitrogen assimilation. Increase in ammonium ion concentration is shown to decrease covalent modification of the Synechococcus PII protein, as in enteric bacteria. We therefore propose that the photosynthetic electron transport chain may regulate the pathway of nitrogen assimilation in cyanobacteria by means of posttranslational, covalent modification of the glnB gene product. The existence of the glnB gene in different strains of cyanobacteria is demonstrated and its implications are discussed.

Original languageEnglish
Pages (from-to)4565-4569
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number11
StatePublished - Jun 1 1991
Externally publishedYes

Fingerprint

Cyanobacteria
Post Translational Protein Processing
Electron Transport
Nitrogen
Synechococcus
Genes
Photosystem I Protein Complex
Photosystem II Protein Complex
Escherichia coli Proteins
Photosynthesis
Enterobacteriaceae
Ammonium Compounds
Signal Transduction
Electrons
Ions
Escherichia coli
Amino Acids
Peptides
Proteins

Keywords

  • Cloning
  • NH concentration
  • Photosystem
  • Sequencing
  • Uridylylation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product. / Tsinoremas, Nicholas; Castets, A. M.; Harrison, M. A.; Allen, J. F.; Tandeau De Marsac, N.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 11, 01.06.1991, p. 4565-4569.

Research output: Contribution to journalArticle

@article{a4cb5b22d2b944b289f991fef363c81e,
title = "Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product",
abstract = "A glnB gene is identified in the cyanobacterium Synechococcus sp. PCC 7942, and its gene product is found to be covalently modified as a result of imbalance in electron transfer in photosynthesis, where photosystem II is favored over photosystem I. The gene was cloned and sequenced and found to encode a polypeptide of 112 amino acid residues, whose sequence shows a high degree of similarity to the Escherichia coli regulatory protein, PII. In E. coli, PII is involved in signal transduction in transcriptional and post-translational regulation of nitrogen assimilation. Increase in ammonium ion concentration is shown to decrease covalent modification of the Synechococcus PII protein, as in enteric bacteria. We therefore propose that the photosynthetic electron transport chain may regulate the pathway of nitrogen assimilation in cyanobacteria by means of posttranslational, covalent modification of the glnB gene product. The existence of the glnB gene in different strains of cyanobacteria is demonstrated and its implications are discussed.",
keywords = "Cloning, NH concentration, Photosystem, Sequencing, Uridylylation",
author = "Nicholas Tsinoremas and Castets, {A. M.} and Harrison, {M. A.} and Allen, {J. F.} and {Tandeau De Marsac}, N.",
year = "1991",
month = "6",
day = "1",
language = "English",
volume = "88",
pages = "4565--4569",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11",

}

TY - JOUR

T1 - Photosynthetic electron transport controls nitrogen assimilation in cyanobacteria by means of posttranslational modification of the glnB gene product

AU - Tsinoremas, Nicholas

AU - Castets, A. M.

AU - Harrison, M. A.

AU - Allen, J. F.

AU - Tandeau De Marsac, N.

PY - 1991/6/1

Y1 - 1991/6/1

N2 - A glnB gene is identified in the cyanobacterium Synechococcus sp. PCC 7942, and its gene product is found to be covalently modified as a result of imbalance in electron transfer in photosynthesis, where photosystem II is favored over photosystem I. The gene was cloned and sequenced and found to encode a polypeptide of 112 amino acid residues, whose sequence shows a high degree of similarity to the Escherichia coli regulatory protein, PII. In E. coli, PII is involved in signal transduction in transcriptional and post-translational regulation of nitrogen assimilation. Increase in ammonium ion concentration is shown to decrease covalent modification of the Synechococcus PII protein, as in enteric bacteria. We therefore propose that the photosynthetic electron transport chain may regulate the pathway of nitrogen assimilation in cyanobacteria by means of posttranslational, covalent modification of the glnB gene product. The existence of the glnB gene in different strains of cyanobacteria is demonstrated and its implications are discussed.

AB - A glnB gene is identified in the cyanobacterium Synechococcus sp. PCC 7942, and its gene product is found to be covalently modified as a result of imbalance in electron transfer in photosynthesis, where photosystem II is favored over photosystem I. The gene was cloned and sequenced and found to encode a polypeptide of 112 amino acid residues, whose sequence shows a high degree of similarity to the Escherichia coli regulatory protein, PII. In E. coli, PII is involved in signal transduction in transcriptional and post-translational regulation of nitrogen assimilation. Increase in ammonium ion concentration is shown to decrease covalent modification of the Synechococcus PII protein, as in enteric bacteria. We therefore propose that the photosynthetic electron transport chain may regulate the pathway of nitrogen assimilation in cyanobacteria by means of posttranslational, covalent modification of the glnB gene product. The existence of the glnB gene in different strains of cyanobacteria is demonstrated and its implications are discussed.

KW - Cloning

KW - NH concentration

KW - Photosystem

KW - Sequencing

KW - Uridylylation

UR - http://www.scopus.com/inward/record.url?scp=0025727050&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025727050&partnerID=8YFLogxK

M3 - Article

VL - 88

SP - 4565

EP - 4569

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -