Abstract
Human retinal gene 4 (HRG4) (UNC119) is a photoreceptor synaptic protein of unknown function, shown when mutated to cause retinal degeneration in a patient and in a confirmatory transgenic model. ADP-ribosylation factor-like protein 2 (ARL2) was identified as an interactor of HRG4 by the yeast two-hybrid strategy. The presence of ARL2 in the retina and co-localization with HRG4 was confirmed by Western blot and double immunofluorescence analysis, respectively. The interaction of ARL2 with HRG4 was further confirmed by co-immunoprecipitation and direct binding analysis. Phosphodiesterase δ (PDEδ) is an ARL2-binding protein homologous to HRG4. Amino acid residues of PDEδ involved in binding ARL2 and forming a hydrophobic pocket were shown to be highly conserved in HRG4, suggesting similarity in binding mechanism and function.
Original language | English (US) |
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Pages (from-to) | 26-32 |
Number of pages | 7 |
Journal | FEBS letters |
Volume | 534 |
Issue number | 1-3 |
DOIs | |
State | Published - Jan 16 2003 |
Keywords
- ADP-ribosylation factor-like protein 2
- Human retinal gene 4
- Interactor
- Phosphodiesterase δ
- UNC119
- Yeast two-hybrid
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology